Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the cell membranes are either removed (imt and smtp) or mechanically (thom) and chemically permeabilized (pfi), while the mitochondrial functional integrity and to a large extent the mt-structure is maintained. According to this definition, submitochondrial particles (smtp) are not a mtprep.
MitoPedia topics: Sample preparation
Communicated by Gnaiger E 2011-11-15, edited 2017-04-20.
- » Compare: Submitochondrial particles, smtp
- O2k-Protocols for mt-preparations: O2k-mitochondrial preparations
|BEC 2020.1 doi10.26124bec2020-0001.v1||Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1||2020|
Preparation: Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate