- MitoPedia - high-resolution terminology - matching measurements at high-resolution.
The MitoPedia terminology is developed continuously in the spirit of Gentle Science.
|Blood cell preparation||bcp||Blood cell preparation (bcp) is one of the key steps in diagnostic protocols.|
|Blood plasma||Plasma||Blood plasma is the non-cellular component of the blood. Plasma lacks cellular components of the blood, RBC, WBC and platelets. However, there are many proteins in plasma, i.e. fibrinogen, albumin and globulin. Both blood plasma and PRP maintain clotting activity after whole blood separation.|
|Blood serum||Serum||Blood serum is a purified plasma in which the coagulant components were removed from the blood plasma. It contains other substances, i.e. antibodies, antigens and hormones. Serum can be obtained by collecting the liquid phase after blood or plasma coagulation.|
|Isolated mitochondria||imt||Isolated mitochondria, imt, are mitochondria separated from a tissue or cells by breaking the plasma membranes and attachments to the cytoskeleton, followed by centrifugation steps to separate the mitochondria from other components.|
|Living cells||vce||Cell viability in living cells should be >95% for various experimental investigations, including cell respirometry. Viable cells (vce) are characterized by an intact plasma membrane barrier function. The total cell count (Nce) is the sum of viable cells (Nvce) and dead cells (Ndce). In contrast, the cell membrane of cells can be permeabilized selectively by mild detergents (digitonin), to obtain the mt-preparation of permeabilized cells used for cell ergometry. Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest the alternative meaning of viable or unaffected by a disease or mitochondrial injury.|
|Mitochondrial concentration||CmtE||Mitochondrial concentration is CmtE = mtE·V-1 [mtEU·m-3]. mt-Concentration is an experimental variable, dependent on sample concentration.|
|Mitochondrial content||mtENX||Mitochondrial content per object X is mtENX = mtE·NX-1 [mtEU·x-1].|
|Mitochondrial density||DmtE||Specific mitochondrial density is DmtE = mtE·mX-1 [mtEU·kg-1]. If the amount of mitochondria, mtE, is expressed as mitochondrial mass, then DmtE is the mass fraction of mitochondria in the sample. If mtE is expressed as mitochondrial volume, Vmt, and the mass of sample, mX, is replaced by volume of sample, VX, then DmtE is the volume fraction of mitochondria in the sample.|
|Mitochondrial preparations||mtprep||Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the cell membranes are either removed (imt and smtp) or mechanically (thom) and chemically permeabilized (pfi), while the mitochondrial functional integrity and to a large extent the mt-structure is maintained. According to this definition, submitochondrial particles (smtp) are not a mtprep.|
|Permeabilized cells||pce||Permeabilized cells (pce) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane (e.g., with digitonin), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Permeabilized cells (pce) are, therefore, not any longer viable or living cells (ce), since the intactness of cells implies the intactness of the plasma membrane. Any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells.For permeabilizing the cell plasma membranes chemically with digitonin, without damaging the mt-membranes, the optimum concentration of digitonin must be previously determinated. The protocol SUIT-010 is designed for the evaluation of optimum digitonin concentration for permeabilizing cells, a requirement to account for differences between cell types, the concentration of cells, and variability between batches of the natural product digitonin.
|Permeabilized muscle fibers||pfi||Permeabilized muscle fibers (pfi) are used as a mitochondrial preparation in respirometry to access mitochondrial function comparable to isolated mitochondria (imt). pfi are obtained by selectively permeabilizing the plasma membrane mechanically and chemically (saponin), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
|Permeabilized tissue||pti||Permeabilized tissue (pti, see also permeabilized muscle fibers, pfi) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically (e.g., with saponin), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.|
|Platelet-rich plasma||PRP||Platelet-rich plasma (PRP) is obtained as the upper layer at low-speed centrifugation (around 150-200 g), when white and red blood cells sediment and thus get separated from plasma containing the platelets. For further details see blood cell preparation.|
|Sample||s, S||A sample is one or more parts taken from a study group of objects, either stored for later studies or prepared and possibly separated into subsamples, which are enclosed in a system for investigation. A pure sample S is a pure gas, pure liquid or pure solid of a defined elementary entity. Then the system used to study sample S contains only entities of type S, and the volume VS [L] and mass mS [kg] of the (sub)sample S are identical to the volume V and mass m of the system. A pure sample S may be mixed with other components to be studied as a mixture, and (sub)sample s is investigated in combination with other components in a system, such that the volume Vs [L] and mass Ms [kg] of the (sub)sample s are smaller than the volume V and mass m of the system. Clarity of statistical representation is improved, if the symbol N is used for the number of primary samples taken from a study group, and the symbol n is used for the number of subsamples studied as technical repeats.|
|Sample mass concentration||Cms||Sample mass concentration is Cms = ms·V-1 [kg·m-3].|
|Submitochondrial particles||smtp||Submitochondrial particles (smtp) consist of membrane fragments which retain most of the enzymatic machinery required in electron transfer and oxidative phosphorylation. Such membrane fragments are continuous closed vesicles formed by resealing of mt-membrane fragments after disruption of the mitochondrial structure. smtp are used to isolate the inner-membrane-bound ET-pathway (mETS) from the upstream modules of the Electron transfer-pathway (ETS) which are located in the mt-matrix and outer mt-membrane (transporters). smtp are obtained by treatment of mitochondria with membrane-dispersing agents such as digitonin at high concentration or by sonic irradiation.|
|Tissue homogenate||thom||A tissue homogenate (thom) is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized.|