O2k-Open Support alert

O2k-Open Support alert

Currently Answered Questions, CAQ, from the »Oroboros Ecosystem Agenda«

Mar 2020

Mar-30 Mo

DatLab 7.4 offers the flexibility to edit a DL-Protocol: »Enable DL-Protocol editing«

Mar-25 We

O₂ flux analysis with DatLab 7.4. Use our latest O₂ analysis template: »O2k-Videosupport: O₂ flux analysis with DatLab 7.4«

Mar-23 Mo

New

Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O₂ background on the O2k-sV-Module?

(1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O₂ background can be performed with the "BG Feedback" settings. - »O2k-sV-Module«

(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O₂ background can be performed in the O2k-sV-Module. - »O2k-sV-Module«

Mar-11 We

New

Do you change the slope smoothing to 20 while running experiments with biological sample?

While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »Slope smoothing«

Mar-04 We

New

A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«

Feb 2020

Feb-28 Fr

New

Calibration parameters are transferred to experimental DatLab files

Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«

Jan 2020

Jan-31 Fr

Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration

In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »Oxygen calibration - DatLab«

Jan-30 Th

Do you check the O₂ flux at the end of the air calibration with the O2k-chamber closed?

In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O₂ slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »Oxygen calibration - DatLab«

Do you set mark ‘R1’ and complete the air calibration correctly?

Your air calibration is not effective until you set mark ‘R1’ and click on Calibrate and copy to clipboard in the O2 calibration window. - »Oxygen calibration - DatLab«

Jan-24 Fr

Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?

Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. »O2k configuration«

Do you perform O2k-Chamber volume calibration?

The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«

Do you use the correct O₂ solubility factor (F_M) in the ‘O2 calibration’ window in DatLab?

The O₂ solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«