What is the advantage of using flux control ratios (FCRs)?
FCRs provide information on coupling and pathway control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) determination of tissue mass or mt-markers.
- »Flux control ratio«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«
Do you know how to export a user-specific DL-protocol (*.DLPU)?
Do I need to calibrate the fluorescence signal with safranin?
It is possible to run the experiment without calibration, presenting only the fluorescence intensity plot. However, a simple two-point or multiple-point calibration for safranin concentration is possible.
- »Safranin calibration«
Simultaneous fluctuations in O2 slope neg. and the signal of the Peltier power?