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O2k-OpenSupportAlert2023 Archive

From Bioblast
Answered Questions from the »Oroboros Ecosystem Agenda 2023 Archive«

Dec 2023

  • Dec- 27 We
O2k-Open SupportInfo: improve Do you observe a high signal at zero oxygen?

If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.
- »Leak current test«
- communicated by »Baglivo Eleonora« and »Grings Mateus«

  • Dec- 11 Mo - International Mountain Day
O2k-Open SupportInfo: improve Mountain slopes can be difficult, but O2 slopes can be smooth with DatLab

The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
- »Smoothing in Flux/Slope«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

  • Dec- 05 Tu
O2k-Open SupportInfo: improve Is DatLab 8 compatible with your operative system?

The DatLab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
- »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Nov 2023

  • Nov- 20 Mo
O2k-Open SupportInfo: improve Do I need to change the filter set of the O2k-Fluo Smart-Module?

Because two different filter sets can be used with the blue Fluo-Sensors, filters should be changed according to the fluorophore that will be used. Extra filters are provided for replacement in case of damage.
- »O2k-Fluo Smart-Module manual« and »O2k-Fluo Smart-Module - Fluorescence dyes and settings« 
- communicated by »Cardoso Luiza« and »Timon-Gomez Alba«

  • Nov- 07 Tu
O2k-Open SupportInfo: take care How often should I re-assemble the O2k-chamber?

We recommend disassembling, cleaning and re-assembling the O2k-chamber at least every 1-2 years. It is also necessary to disassemble it when there is persistent biological or protein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.
- »O2k-chamber assembly - possible problems« and »O2k-Videosupport: Insert O2k-chamber«
- communicated by »Timon-Gomez Alba« and »Schmitt Sabine«

Oct 2023

  • Oct- 17 Tu
O2k-Open SupportInfo: improve Noticing higher values of J°1 when using the O2k-Chamber sV (0.5 mL) compared to the 2 mL O2k-Chamber?

The instrumental oxygen background flux, J°O2, is volume-dependent in the closed chamber; therefore, the values of J°1 are about four times higher in the sV chamber.
- »O2k-sV-Module«, »POS calibration-SOP«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«

Sep 2023

  • Sep- 11 Mo
O2k-Open SupportInfo: improve Barometric pressure displaying as 0 kPa in the O2 calibration window?

In many cases, this can be solved by by switching off and unplugging the O2k for several minutes.
- »O2k-Barometric Pressure Transducer«
- communicated by »Schmitt Sabine« and »Grings Mateus«

Aug 2023

  • Aug- 28 Mo
O2k-Open SupportInfo: improve Can I compare data sets recorded with different sensor gain settings?

Oxygen signals obtained after proper calibration are independent of the gain settings, therefore oxygen concentrations (and fluxes) can be compared, as long as the corresponding air and zero calibrations are applied.
- »Gain of the O2 channel«
- communicated by »Baglivo Eleonora« and »Leo Elettra«

  • Aug- 14 Mo
O2k-Open SupportInfo: improve What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?

It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings.
- » Troubleshooting for problems with text in DatLab«
- communicated by »Cecatto Cristiane« and »Timon-Gomez Alba«

Jul 2023

  • Jul- 17 Mo
O2k-Open SupportInfo: take care Are you planning to perform several measurements in one day using the same O2k-chamber?

To avoid any possible contamination by inhibitors in the subsequent measurements, the O2k-chamber needs to be cleaned each time with the standard protocol AfterUse or AfterUse_inhibitors.
- »O2k-cleaning - Sections 2.3 and 2.4«
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«

May 2023

  • May- 09 Tu
O2k-Open SupportInfo: take care Does your polarographic oxygen sensor (POS) need a deep clean?

A POS with a very dark anode might need several rounds of ammonia cleaning, possibly including prolonged exposure overnight to restore performance specifications.
- »POS service«
- communicated by »Cecatto Cristiane« and »Leo Elettra«

Mar 2023

  • Mar- 28 Tu
O2k-Open SupportInfo: improve Unusual stirrer bar rotation?

A “jumping” stirrer or a slow rotation of the stirrer bar may indicate a problem either with the stirrer itself or with the cleaning of the glass chamber.
- »Stirrer bar - technical support«
- communicated by »Baglivo Eleonora« and »Grings Mateus«

  • Mar- 21 Tu
O2k-Open SupportWarning: loss of data Did you know that a humid environment and/or liquid spillage may lead to a high raw POS signal [V]?

See how to clean and dry the electrical connections of the OroboPOS-Connector.
- »Cleaning the electrical connections«
- communicated by »Cecatto Cristiane« and »Baglivo Eleonora«

Feb 2023

  • Feb- 23 Th
O2k-Open SupportInfo: improve Add catalase when measuring Complex IV (CIV) activity in permeabilized fibers (pfi)

Pfi have a high content of iron-containing proteins, which accelerate the autoxidation of tetramethyl-p-phenylenediamine (TMPD) in the CIV assay. Catalase prevents the accumulation of H2O2 generated by this autoxidation.
- »Chemical Background« and »Complex IV«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«

  • Feb- 16 Th
O2k-Open SupportInfo: improve Do you observe simultaneous fluctuations in the O2 slope neg. and the Peltier power?

This can indicate fluctuations in the room temperature or electrical supply. Controlling the temperature or changing the electrical supply may resolve this problem.
- »Troubleshooting«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«