MiPNet09.12 O2k-Titrations
O2k manual titrations: SUIT protocols with mitochondrial preparations. |
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Oroboros (2020-08-17) Mitochondr Physiol Network
Abstract: Oroboros (2020) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(20):1-4.
- Β» Current O2k-series: NextGen-O2k Series XB and O2k Series J
- Β» Current software versions DatLab 8.0: MitoPedia: DatLab
- Further details: Β» MitoPedia: O2k-Open Support
- Β» O2k-Protocols
- Β» Product: Oroboros O2k, O2k-Catalogue
β’ O2k-Network Lab: AT_Innsbruck_Oroboros
Correction
Oligomycin: titrations should be performed by 1-2 Β΅L steps (i.e. 5-10 nM experimental concentration of each step). Careful titration of oligomycin is necessary to determine the optimal inhibitory concentration (from 10 to 40 nM) as elaborated in Vernerova et al 2021 and Zdrazilova et al 2022 and to prevent inhibition of ET respiration as detailed in Ruas et al (2016) and Doerrier et al 2018.
Labels: MiParea: Instruments;methods
Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid
HRR: O2k-Protocol
O2k-chemicals and media, ADP