MiPNet09.12 O2k-Titrations

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MiPNet09.12 O2k-Titrations

Publications in the MiPMap
O2k-Protocols
O2k manual titrations: SUIT protocols with mitochondrial preparations.

Β» Bioblast pdf Β»Versions

Oroboros (2020-08-17) Mitochondr Physiol Network

Abstract: Oroboros (2020) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(20):1-4.

O2k-technical support and open innovation
Open the pdf document above.
Β» Current O2k-series: NextGen-O2k Series XB and O2k Series J
Β» Current software versions DatLab 8.0: MitoPedia: DatLab
Β» O2k-Protocols
Β» Product: Oroboros O2k, O2k-Catalogue


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros


Correction

Oligomycin: titrations should be performed by 1-2 Β΅L steps (i.e. 5-10 nM experimental concentration of each step). Careful titration of oligomycin is necessary to determine the optimal inhibitory concentration (from 10 to 40 nM) as elaborated in Vernerova et al 2021 and Zdrazilova et al 2022 and to prevent inhibition of ET respiration as detailed in Ruas et al (2016) and Doerrier et al 2018.


Labels: MiParea: Instruments;methods 




Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid 


HRR: O2k-Protocol 

O2k-chemicals and media, ADP 

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