From Bioblast
MiPNet28.11 IOC163 Schroecken AT
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Oroboros (2024-06-17) Mitochondr Physiol Network
Abstract: Oroboros O2k-Workshop on high-resolution respirometry. Schroecken, Austria (2024 June 17-22).
• O2k-Network Lab: AT_Innsbruck_Oroboros
Feedback
- Please help us to improve the O2k-Workshops by taking a few minutes to answer the following questions: IOC163 Feedback
- Perfect hands on training with proper explanation of the experiment. Discussion and troubleshooting of the experiment. - Kumar Premranjan
- What I liked most was the atmosphere of familiarity and serenity and the high level of professionalism and knowledge of the tutors. Even though I was already using the O2kOroboros to assess thr cellular respiration, I realized that there are several steps in which I can improve the performance of my instrument. The topic that I was very interested in and that satisfied me but I would like to address again (if possible) in a future workshop is the use of fluorescent probes with the resulting data analysis. Fantastic experience! - Cerioni Liana
- It was a good balance of theory and hands-on. I liked how each of our groups got to analyze our own data and dicuss it with the entire group and receive feedback. - Viswanathan Dhivya
- The workshop delivered way beyond my expectations, in terms of the balance between lectures & hands-on sessions, the quality of tutoring, and an environment with only collaboration and no competition (and SO MUCH fun!), and I must commend the organizing team for that. - Nizami Hina Lateef
- The workshop in Schroecken was fantastic - you have a great team. - Ricks Rachel
- The workshop was incredible. It was a good mix of presentation and hands-on experience. Meeting the Oroboros team was fantastic. They were helpful and open to all of our questions. It was also great meeting the other participants. All-in-all a 5 out of 5 experience in a beautiful setting! - Haussmann Mark
| Question | Rating (average) 1-5 |
|---|---|
| In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). | 4.7 |
| Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). | 3.2 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: | 4.7 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: | 4.9 |
| Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). | 3.4 |
| Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). | 5 |
| Intensity of the O2k-Course: Please rate from 1 (I felt overwhelmed) to 5 (I would have liked to do and hear more). | 3.6 |
| Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). | 4.9 |
General information
- This training course on the Oroboros O2k and NextGen-O2k provides an introduction to DatLab 8 and applications of the TIP2k. Demos and hands-on experiments illustrate the principle and show the unique advantages and limitations of simultaneous monitoring of oxygen concentration and mitochondrial respiration. The O2k-Fluo Smart-Module is presented with hands-on calibrations for Amplex UltraRed (hydrogen peroxide, ROS) or safranin (mitochondrial membrane potential). Many optimized SUIT protocols are available as DL-Protocols. The SUITbrowser helps you find the best SUIT protocol for your specific research questions. Furthermore, this workshop covers interpretation as well as calibration and instrumental service.
Delays
- We would like to advise you to make sure to book flights that will allow you to arrive in Bregenz in time for the scheduled pickup.
- In the case of delay, please call Oroboros Instruments Mobile: +43 677 64 79 29 17 or Oroboros Office: +43 512 566 796 (until 5:30 pm) as soon as you know you will arrive late.
- If you miss the scheduled transportation you will have to take a cab (Suggestion: Ritter Transport. Phone number: +43 664 34 11 540) or the public transportation to Schroecken. If you decide to take the public transportation, you will have to take the bus via Bregenz- Egg – Bezau – Schoppernau – Schröcken Dorf. You can book the ticket here: vmbol or here: oebb. Please note that the last bus leaves Bregenz at 17:33, after that the only option to get to Schroecken is by cab.
- Please be aware that you will be responsible for paying for any additional transportation costs (about € 120 for a cab/ travel time 1 h; about € 11 for the public transportation / travel time 1.5 h - 2 h). Please check with your airline to see if there are any reimbursements available in the event that you miss the organized transport and have to arrange and pay for a cab or public transportation.
Program
- Program found here:
- Program found here:
Tutors and lecturers
Luiza Cardoso, PhD, Oroboros Instruments - Mitochondrial Wizard
Erich Gnaiger, PhD, Oroboros Instruments - CEO, Innovation Alchemist
Mateus Grings, PhD, Oroboros Instruments, Mitochondrial Jedi
Elettra Leo, PhD, Oroboros Instruments, Mitochondrial Mermaid
Jaime Willis, PhD, Oroboros Instruments, PostDoc
Participants
| Participant | Institution | ||
|---|---|---|---|
| 1. | 
|
Zhang Peng | Ocean State Research Institute at Providence VA Healthcare System, US |
| 2. | 
|
Viswanathan Dhivya | IN Chennai Labmate - Labmate, IN |
| 3. | 
|
Blacksburg, US | |
| 4. |
|
Blacksburg, US | |
| 5. | 
|
Haussmann Mark | US PA Lewisburg Haussmann M - Bucknell University, US |
| 6. | 
|
Lichtner Kayla | US PA Lewisburg Haussmann M - Bucknell University, US |
| 7. | 
|
Cerioni Liana | IT Urbino Cerioni L - Dep. of Biomolecular Sciences, University of Urbino, IT |
| 8. |  |
Laizāne Linda | DK Copenhagen Dela F - Riga Stradins University, LV |
| 9. | 
|
Nizami Hina Lateef | US OK Oklahoma City Miller BF - Oklahoma Medical Research Foundation, US |
| 10. |
|
Kumar Premranjan | Baylor College of Medicine, US |
| 11. | 
|
Ricks Rachel | US UT Provo Bikman BT - Brigham Young University, US |
| 12. |
|
Paszko Adam | Medical University of Bialystok, PL |
| 13. |
|
Golonko Aleksandra | Medical University of Bialystok, PL |
| 14. |
|
Konopka Paulina | Medical University of Bialystok, PL |
| 15. | 
|
Boltman Taahirah | ZA Cape Town Maarman GJ - Stellenbosch University, ZA |
| 16. | 
|
Mokoena Kheya | ZA Cape Town Maarman GJ - Stellenbosch University, ZA |
| 17. | 
|
Clayton Sally | UK Birmingham Lai YC - University of Birmingham, UK |
| 18. |
|
Aurora, US | |
| 19. | 
|
Dantas Wagner S | US LA Baton Rouge Noland RC - Pennington Biomedical Research Center, US |
| 20. | 
|
Bakkeren Ciska | DK Aarhus Fago A - Aarhus University, DK |
| 21. | 
|
DeStephanis Darla | US NC Winston-Salem Kramer PA - Wake Forest Baptist Health, US |
| 22. |
|
Kingren Meagan | US AR Little Rock Borsheim E - University of Arkansas for Medical Sciences, US |
| 23. | 
|
Herpe Lea | CA Moncton Pichaud N - University of Moncton, CA |
| 24. |
|
Fuior Elena Valeria | Institute of Cellular Biology and Pathology, RO |
| 25. |
|
Multescu Marius | Institute of Cellular Biology and Pathology, RO |
| 26. |
|
Wieser-Insole Joshua | AT Innsbruck Oroboros - VASCage GmbH, AT |
O2k perspectives - abstracts
- Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.
- » Abstract guidelines
| Has title | |
|---|---|
| Mokoena 2024 Abstract IOC163 | Mokoena KR, Webster I, Genis A, Boltman T, Cambell J, Holm J, Mbombela V, Adoga JO, Everson FP, Maarman GJ, Strijdom H (2024) Investigating mitochondrial function in HIV-associated cardiometabolic disease: preliminary findings from a South African population. Mitochondr Physiol Network 28.11. |
| Dantas 2024 Abstract IOC163 | Dantas WS, Heintz EC, Zunica ERM, Dousay M, Davuluri G, Yu CS, Murphy MP, Axelrod CL, Kirwan JP (2024) Mito-Q supplementation reverses sarcopenic obesity in aged mice by restoring mitochondrial function and enhancing antioxidant response. Mitochondr Physiol Network 28.11. |
| Bakkeren 2024 Abstract IOC163 | Bakkeren C, Hejlesen R, Kjær-Sørensen K, Oxvig C, Malte H, Fago A (2024) Multilevel physiological analysis of myoglobin knockout zebrafish. Mitochondr Physiol Network 28.11. |
| Clayton 2024 Abstract IOC163 | Clayton SA, Daley KK, O’Neil JD, MacDonald L, Mahida R, Pitceathly RDS, Kurowska-Stolarska M, Fernandez-Vizarra E, Dimeloe S, Clark AR (2024) Investigating the role of the NDUFA4 (COXFA4) family of electron transport chain proteins in inflammation. Mitochondr Physiol Network 28.11. |
| Herpe 2024 Abstract IOC163 | Herpe L, Pichaud N (2024) Investigating the importance of mtG3PDH in mitochondrial flexibility during environmental and physiological changes. Mitochondr Physiol Network 28.11. |
| Nizami 2024 Abstract IOC163 | Nizami HL, Szybowska P, Singhal P, Lee CF (2024) Investigating the effect of Nampt overexpression on cardiac mitochondrial respiration in Ndufs4-cKO mice. Mitochondr Physiol Network 28.11. |
| ... further results | |
Impressions from the IOC
IOC163:
Group picture IOC163
Presentations at IOC163
Erich with participants at IOC163
Syringe cleaning at IOC163
Data analysis at IOC163
Hands-on training at IOC163
Hands-on training at IOC163
Winners of the quiz at IOC163
Walk and Talk:
Koerbersee
Walk and Talk
Walk and Talk
Walk and Talk
The Oroboros-Team at IOC163
Hotel Koerbersee
Walk and Talk at Koerbersee
Visit at the Alpmuseum
Venue
- Hotel Körbersee, Schröcken, AT
- Information on travel and venue: IOC Schroecken.
- » As there is no ATM close to the hotel, we recommend withdrawing money at Bregenz train station. Please make sure to bring some cash in EUROs with you, in case you need it.
- The hotel accepts payment by credit card for consumed drinks.
- » As there is no ATM close to the hotel, we recommend withdrawing money at Bregenz train station. Please make sure to bring some cash in EUROs with you, in case you need it.
- » Please note that the hotel has a limited number of rooms and space is not guaranteed. Therefore, we ask that you to confirm with us in advance if you intend to bring accompanying guests with you.
Impressions from Hotel Koerbersee:
Hotel Koerbersee © Berghotel Körbersee
Hotel Koerbersee Room Enzian © Berghotel Körbersee
Hotel Koerbersee Room Schneestern © Berghotel Körbersee
Koerbersee
Registration
- Registration closed. IOC163 is fully booked.
- Registration includes: Coffee breaks and transportation from Bregenz to Schroecken and back
Contact
- Oroboros Instruments
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 677 647 929 17
- Fax: +43 512 566796 20
- Mitochondria and Cell Research
IOC recommended reading
- The Must-Read for the O2k-Workshop:
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. - »Bioblast link« - A new edition with updated terminology and more ..
- » Chapter 2: Respiratory states and rates (estimated reading time 30 min – 1 h)
- » Chapters 4-5: NADH-linked pathways through Complex CI and S-pathway through Complex CII (estimated reading time 30 min – 1 h)
- » Chapter 6: Convergent electron transfer at the Q-junction (estimated reading time 45 min – 1 h 30 min)
- Further reading:
- » IOC recommended reading
- » MitoPedia: Respiratory states
O2k-Workshops are listed as MitoGlobal Events.
Labels:
ORO, IOC, MitoFit, 2024, MitoGlobal, NextGen-O2k, O2k-Network Award
