Talk:O2k-Videosupport

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Last update: 2018-04-05

Videoclip for pfi

Last update: 2018-04-05

  1. We will need some new sequences to integrate in the videoclips: we will not use liquid into the cup for taking the wet weight.

Last update: 2018-02-12

  1. 02:00 --> When BIOPS is added to the wells (plate): add the text: all wells 2 mL BIOPS. The text will stay until: 02:10.
  2. 02:18 --> We add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL (instead of the 2 mL pippete). We add 1 mL of BIOPS to the Petridish as well.
  3. 03:00 --> Add text for the saponin concentration --> 5 mg/mL in BIOPS
  4. 03:37 --> Add text with the information --> 20 µL saponin in 2 mL BIOPS
  5. 03:42 --> Add text with the information --> BIOPS (this text will be in the first line of the plate); BIOPS+saponin (in the second line); BIOPS (in the last line)
  6. 04:41 --> Incorporate images from the binocular. Johannes: is maybe possible to do any animation (with the panel showing all the steps)?
  7. 06:49 --> Add the text: Aluminum cup on the petri dish
  8. 07:50 --> Add the text: Blotting during 40 seconds
  9. 08:13 --> Add to the video (if Johannes took it, I think he took it. Checking if the forceps are dry after taking the wet weight) --> and add the text: check the forceps
  10. 08:16 --> Add the text info?: take wet weight
  11. ~08:39-08:40 --> When we take the forceps, we can see something.
  12. ~08:56-08:59 --> Add the text: Add O2


Clips for pfi

Last update: 2018-02-12

1. Materials (00:00-01:30)
2. BIOPS and saponin (01:30-03:55)
3. Muscle fibre preparation: Mechanical Preparation (03:55-04:57)
4. Muscle fibre preparation: Chemical Permeabilization (04:57-06:33)
5. Wet Weight (06:34-08:17)
6. To the O2k (08:18-09:15)


Videoclip for Shredder

Last update: 2018-04-05

  1. We will need some new sequences to integrate in the videoclips: we will not use liquid into the cup for taking the wet weight.

Last update: 2018-02-12

  1. ~00:19 --> Add text? Calculate volume needed for your thom (here, pipette adding 5 mL, show pipette 5 mL (00:41)).
  2. 00:41 --> Not 5 mL here, show pippete with 500 µL.
  3. 01:38 --> Could we incorporate a video taking the wet weight from the fiber videoclip? It will be added to the Shredder videoclip (01:38, before we see the eppendorf tube with the yellow label). We can take the video-section from the fibres (To include the sequences: 06:58-07:06; 07:12-07:13 maybe here you could do a zoom showing only the Falcon tube with the MiR05-Kit; 07:17-07:21; 07:31-07:37; 07:45-08:12; include the section checking the forceps from the fibres from 08:13; 08:19-08:20. Finally show the sequence 01:39 in the Shredder clip).
  4. ~02:06 --> Text: Cut into smaller pieces
  5. ~02:21 --> Text? Distributed on the Lysis Disk
  6. ~04:02 --> 5 seconds instead of 10 seconds (in the position 2)
  7. ~07:33--> Not all the volume is take it. Does Johannes has another video?
  8. Last part of the video is in the pfi video.


Clips for Shredder

Last update: 2018-02-12

1. Materials (00:00-01:30)
2. Tissue preparation (01:30-03:02)
3. Homogenization (~03:02-04:27)
4. Retrieval (~04:27-to the end)
5. To the O2k (from 09:12 in the pfi video)


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