Xue 2019 Sci Rep
Xue YP, Kao MC, Lan CY (2019) Novel mitochondrial complex I-inhibiting peptides restrain NADH dehydrogenase activity. Sci Rep 9:13694. |
Xue YP, Kao MC, Lan CY (2019) Sci Rep
Abstract: The emergence of drug-resistant fungal pathogens is becoming increasingly serious due to overuse of antifungals. Antimicrobial peptides have potent activity against a broad spectrum of pathogens, including fungi, and are considered a potential new class of antifungals. In this study, we examined the activities of the newly designed peptides P-113Du and P-113Tri, together with their parental peptide P-113, against the human fungal pathogen Candida albicans. The results showed that these peptides inhibit mitochondrial complex I, specifically NADH dehydrogenase, of the electron transport chain. Moreover, P-113Du and P-113Tri also block alternative NADH dehydrogenases. Currently, most inhibitors of the mitochondrial complex I are small molecules or artificially-designed antibodies. Here, we demonstrated novel functions of antimicrobial peptides in inhibiting the mitochondrial complex I of C. albicans, providing insight in the development of new antifungal agents.
β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Fungi
Preparation: Intact cells Enzyme: Complex I
HRR: Oxygraph-2k
Labels, 2019-10