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• O2k-Fluo Smart-Module
  == Mounting a filter cap == :::* ''See'': [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a filter cap]]
  6 KB (824 words) - 07:14, 29 March 2024
• O2k-Fluo LED2-Module
  == Mounting a filter cap == :::* ''See'': [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a filter cap]]
  6 KB (848 words) - 07:02, 29 March 2024
• Time resolution
  ...lux. While the on-line filter in DatLab is fixed (Savitzky-Golay smoothing filter applied on the 40 proceeding data points), DatLab 2 options are many. ...ependent of the absolute oxygen pressure. In the scientific literature, it has been argued that at very low oxygen the time constant increases - without a
  4 KB (661 words) - 05:52, 19 July 2022
• Rustan 2017 Abstract MITOEAGLE Barcelona
  ...ribed experimental approaches and uses standard 96-well tissue culture and filter plates. The accessibility and high throughput of the described ''in vitro''
  4 KB (536 words) - 14:31, 12 January 2018
• MiPNet26.09 FAT4BRAIN Advanced O2k-Workshop IOC150 Virtual
  ...https://www.youtube.com/watch?v=eUukvgAi1yY O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor] This project has received funding from the European Union's Horizon 2020 research and innova
  4 KB (466 words) - 10:56, 11 April 2022
• Fluorescence-Control Unit
  ...k-Guide: Setup - next step - [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]''' :::: Limitations: For Series D oxygraphs the LED intensity has to be set to the same value for both chambers.
  14 KB (1,881 words) - 10:49, 4 August 2021
• Rhodamine 123
  ::::# Fluo-Sensor: [[Smart Fluo-Sensor Blue]] equipped with [[Filter Set Saf]] ...e optimum concentration of Rhodamine 123 to measure the membrane potential has to be tested in previous experiments.
  5 KB (698 words) - 15:50, 16 March 2021
• TMRM
  ...Fluo-Sensor: [[Fluorescence-Sensor Green]] equipped with [[Filter_Set_AmR|Filter Set AmR]]. ::::* [[Fluorescence-Sensor Green]], [[Filter_Set_AmR|Filter Set AmR]], light intensity: Amp Polarization voltage = 100, Amp Gain = 1000
  17 KB (2,405 words) - 07:29, 5 April 2024
• Kearney 1960 J Biol Chem
  ...each other by purification on ion exchange resins or by chromatography on filter paper. 5. Evidence for the flavin peptide hypothesis has come from the finding that throughout very extensive purification by a vari
  5 KB (718 words) - 20:57, 31 March 2023
• Oxygen flux - instrumental background
  ...compensation, performed by [[DatLab]]. If no experimental background test has been performed, the system default values are used, which are ''a''°=-2.0 ...time derivative of the oxygen signal, called "O2 slope uncorr." in DatLab, has to be zero. The background-corrected oxygen flux is meaningless for the ope
  19 KB (2,868 words) - 10:11, 22 April 2022
• O2k-OpenSupportAlert2020 Archive
  ...nal (O₂ slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O₂ background test?'''<br> ..._in_instrumental_background_test Unstable 'O2 slope neg.']« and »[[TIP2k Filter Papers]]«
  27 KB (4,026 words) - 11:53, 29 March 2021
• Safranin
  :::# Fluo-Sensor: [[Smart Fluo-Sensor Blue]] equipped with [[Filter Set Saf]] ...requires a specific method for each preparation and protein content, which has to be kept constant in an experimental series <ref> Sumbalova Z, Gnaiger E
  13 KB (1,842 words) - 17:09, 17 January 2024
• Houska Award 2012
  ...and WGT over the last 10 years in the development of the Oxygraph-2k (O2k) has led to its establishment as a sole-source instrument for HRR. This initiate :::: suitable filter. Therefore, only the components necessary for the desired applications have
  29 KB (4,140 words) - 23:00, 14 November 2022
• Magnesium Green
  ...] or [[O2k-Fluo LED2-Module]] selecting the [[Fluorescence-Sensor Blue]]/[[Filter Set MgG / CaG]]. Please see these pages for specific aspects of fluorescenc ...suitable for buffers containing high concentrations of Mg²⁺. It has been used in the Oroboros Laboratories with a modified formulation of [[MiR
  17 KB (2,616 words) - 15:42, 7 February 2024
• Amplex UltraRed
  ...plier is called UltroxRed in the case of Amplex^® UltraRed which has a similar structure to resorufin). The change of emitted fluorescence inten ...serted though the windows of the O2k-Chambers (Hickey et al 2012). The O2k has also been coupled to a fluorescence spectrophotometer with a light guide in
  35 KB (5,265 words) - 15:10, 25 April 2024
• Gnaiger 2019 MitoFit Preprints Editorial
  ...s with a high duplication score are automatically labelled by a plagiarism filter as an alert to readers and authors alike (Citron, Ginsparg 2015). At the st ...(''1'') In contrast to physics and mathematics, where publication in arXiv has become a standard, preprints remain relatively unpopular in biomedical scie
  28 KB (3,945 words) - 08:24, 8 January 2023
• Oroboros Ecosystem Agenda 2024 Archive
  Incorrect placement of TIP2k Filter Papers can lead to liquid accumulation and convective oxygen backdiffusion. ...was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my re
  25 KB (3,611 words) - 08:22, 22 April 2024
• More O2k-Feedback
  ...I was exposed to new techniques in conducting mitochondrial research. This has helped me stay up-to-date with the latest research in the mitochondria fiel ::::* ''It has been a pleasure to work with your Oxygraph-devices during the last 13 years
  40 KB (6,559 words) - 15:22, 21 February 2024
• Oroboros Ecosystem Agenda 2023 Archive
  If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electri |'''Do I need to change the filter set of the O2k-Fluo Smart-Module?'''<br>
  80 KB (11,165 words) - 09:37, 8 January 2024
• Picard 2011 PLoS One
  ...a. … The degree of mitochondrial integrity obtained with current protocols has been questioned,31 with less disruptive approaches involving permeabilized ...ces were normalized to wet weight of manually isolated fibers blotted on a filter paper to remove excess of fluid[17,18]. A recent study showed a relatively
  52 KB (7,111 words) - 14:50, 13 January 2023

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