MiR05: Difference between revisions

Revision as of 00:41, 19 May 2022

high-resolution terminology - matching measurements at high-resolution

MiR05

Description

[[Description::Mitochondrial respiration medium, MiR05, developed for oxygraph incubations of mitochondrial preparations. Respiration of living cells may be assessed in MiR05 by adding pyruvate (P) as an external source. MiR06 = MiR05 + catalase. MiR05Cr = MiR05 + creatine.]]

Abbreviation: MiR05

Reference: [[Info::MiR05-Kit, Gnaiger 2000 Life in the Cold, MiPNet22.10 MiR05-Kit]]

Comparison of respiration media

Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - »Bioblast link« - The original article describing MiR05 in comparison with the composition of mitochondrial and organ preservation solutions.
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - »Bioblast link«
Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - »Bioblast link« - "when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers".
Komlódi T, Sobotka O, Krumschnabel G, Bezuidenhoutz N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. - »Bioblast link«
Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - »Bioblast link«

MiR05-Kit in HRR

For preparation of MiR05, see MiPNet22.10 MiR05-Kit.
The oxygen solubility factor of MiR05 is 0.92.

MitoPedia topics: Media for respirometry

@@ Line 3: / Line 3: @@
 |description='''Mitochondrial respiration medium, MiR05''', developed for oxygraph incubations of [[mitochondrial preparations]]. Respiration of [[living cells]] may be assessed in MiR05 by adding pyruvate (P) as an external source.  [[MiR06]] = MiR05 + catalase.
 [[MiR05Cr]] = [[MiR05]] + creatine.
-|info=[https://www.oroboros.at/index.php/product/mir05-kit/ MiR05-Kit], [[Gnaiger 2000 Life in the Cold]]
+|info=[https://www.oroboros.at/index.php/product/mir05-kit/ MiR05-Kit], [[Gnaiger 2000 Life in the Cold]], [[MiPNet22.10 MiR05-Kit]]
 }}
 == Comparison of respiration media ==
@@ Line 12: / Line 12: @@
 :::# Komlódi T, Sobotka O, Krumschnabel G, Bezuidenhoutz N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. - [[Komlodi_2018_Methods Mol Biol |»Bioblast link«]]
 :::# Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - [[Doerrier 2018 Methods Mol Biol |»Bioblast link«]]
 == MiR05-Kit in HRR ==
-::::* For preparation of MiR05, see [[MiPNet22.10 MiR05-kit]].
+::::* For preparation of MiR05, see [[MiPNet22.10 MiR05-Kit]].
 ::::* The '''[[oxygen solubility factor]]''' of MiR05 is '''0.92'''.