Hervouet 2006 Biochem Biophys Res Commun: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
|authors=Hervouet E, Pecina P, Demont J, Vojtíšková A, Simonnet H, Houštek J, Godinot C | |authors=Hervouet E, Pecina P, Demont J, Vojtíšková A, Simonnet H, Houštek J, Godinot C | ||
|year=2006 | |year=2006 | ||
|journal=Biochem Biophys Res Commun. | |journal=Biochem. Biophys. Res. Commun. | ||
|abstract=Cobalt is often used as a hypoxia mimic in cell culture, because it stabilizes the α subunits of the transcription factor, HIF (hypoxia-inducible factor). We have previously shown that HIF stabilization due to a deficiency of the von Hippel Lindau protein (pVHL) in clear cell renal carcinoma (CRCC) was correlated to a down-regulation of oxidative phosphorylation. To better understand this mechanism, we have used CoCl2 in CRCC expressing stably transfected ''vhl''. We show that, in addition to its effect on HIF-α subunits, CoCl2 prevented the normal processing of the precursor of cytochrome ''c'' oxidase (COX) subunit 4 and induced COX degradation very likely by inhibiting the mitochondrial intermediate peptidase (MIP) that cleaves the COX4 precursor protein. This cobalt-induced MIP inhibition was however not observed in other human mitochondrial precursor sequences as previously predicted from comparison between human and yeast mitochondrial precursor sequences. | |abstract=Cobalt is often used as a hypoxia mimic in cell culture, because it stabilizes the α subunits of the transcription factor, HIF (hypoxia-inducible factor). We have previously shown that HIF stabilization due to a deficiency of the von Hippel Lindau protein (pVHL) in clear cell renal carcinoma (CRCC) was correlated to a down-regulation of oxidative phosphorylation. To better understand this mechanism, we have used CoCl2 in CRCC expressing stably transfected ''vhl''. We show that, in addition to its effect on HIF-α subunits, CoCl2 prevented the normal processing of the precursor of cytochrome ''c'' oxidase (COX) subunit 4 and induced COX degradation very likely by inhibiting the mitochondrial intermediate peptidase (MIP) that cleaves the COX4 precursor protein. This cobalt-induced MIP inhibition was however not observed in other human mitochondrial precursor sequences as previously predicted from comparison between human and yeast mitochondrial precursor sequences. | ||
|keywords=Hypoxia-inducible factor, Cytochrome ''c'' oxidase subunit 4, Cytochrome ''c'' oxidase biogenesis, Mitochondrial precursor processing, Mitochondrial intermediate peptidase, Cobalt chloride, ''Homo sapiens'', [[Saccharomyces cerevisiae]] | |keywords=Hypoxia-inducible factor, Cytochrome ''c'' oxidase subunit 4, Cytochrome ''c'' oxidase biogenesis, Mitochondrial precursor processing, Mitochondrial intermediate peptidase, Cobalt chloride, ''Homo sapiens'', [[Saccharomyces cerevisiae]] | ||
Revision as of 13:51, 30 September 2010
| Hervouet E, Pecina P, Demont J, Vojtíšková A, Simonnet H, Houštek J, Godinot C (2006) Inhibition of cytochrome c oxidase subunit 4 precursor processing by the hypoxia mimic cobalt chloride. Biochem Biophys Res Commun. 344(4):1086-93. |
Hervouet E, Pecina P, Demont J, Vojtíšková A, Simonnet H, Houštek J, Godinot C (2006) Biochem. Biophys. Res. Commun.
Abstract: Cobalt is often used as a hypoxia mimic in cell culture, because it stabilizes the α subunits of the transcription factor, HIF (hypoxia-inducible factor). We have previously shown that HIF stabilization due to a deficiency of the von Hippel Lindau protein (pVHL) in clear cell renal carcinoma (CRCC) was correlated to a down-regulation of oxidative phosphorylation. To better understand this mechanism, we have used CoCl2 in CRCC expressing stably transfected vhl. We show that, in addition to its effect on HIF-α subunits, CoCl2 prevented the normal processing of the precursor of cytochrome c oxidase (COX) subunit 4 and induced COX degradation very likely by inhibiting the mitochondrial intermediate peptidase (MIP) that cleaves the COX4 precursor protein. This cobalt-induced MIP inhibition was however not observed in other human mitochondrial precursor sequences as previously predicted from comparison between human and yeast mitochondrial precursor sequences. • Keywords: Hypoxia-inducible factor, Cytochrome c oxidase subunit 4, Cytochrome c oxidase biogenesis, Mitochondrial precursor processing, Mitochondrial intermediate peptidase, Cobalt chloride, Homo sapiens, [[has publicationkeywords::Saccharomyces cerevisiae]]
Labels:
Stress:Cancer; Apoptosis; Cytochrome c, Genetic Defect; Knockdown; Overexpression
Regulation: Respiration; OXPHOS; ETS Capacity
HRR: Oxygraph-2k, Chemicals; Media, Method
