From Bioblast
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP β 2 ADP).
Abbreviation: Ap5A
MitoPedia topics:
Inhibitor
Application in HRR
- Ap5A: P1,P5-Di(adenosine-5β²) pentaphosphate pentasodium salt Sigma D4022, store at -20 Β°C; molecular weight: 1026.28
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 100 mM stock solution (dissolved in H2O):
- For a 2 mg glass vial: dissolve in 19.49 Β΅L H2O
- Store at -20 Β°C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 Β΅L using a 10 Β΅L syringe (2 mL O2k-Chamber).
- Final concentration: 50 Β΅M.