Azide: Difference between revisions

Latest revision as of 17:28, 16 December 2021

high-resolution terminology - matching measurements at high-resolution

Azide

Description

Sodium azide is an inhibitor of Complex IV/cytochrome c oxidase (CIV, COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02

Application in HRR

Azd: Azide (Sodium azide; NaN₃), Sigma-Aldrich: S2002, store at RT, CAS: 26628-22-8, M = 65.01 g·mol^-1

Hazard statements: H300, H310, H330, H373, H410; fatal if swallowed, fatal in contact with skin, fatal if inhaled, may cause damage to organs through prolonged or repeated exposure (brain), very toxic to aquatic life with long-lasting effects

2021-03: Preparation instructions were updated to take the volume of the solute into account (see Volume of the solute). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.

Preparation of 4 M storage and stock solution (1 mL, dissolved in H₂O) for 2-mL O2k-chamber:

Weigh 260 mg of sodium azide.
Dissolve in 900 µL H₂0.
Divide into 0.5 mL portions.
Store at -20 °C.

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): ≥50 µL using a 100 µL Hamilton syringe.
Final concentration: ≥100 mM.

Preparation of 2 M stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

Take 400 µL of the 4 M stock solution.
Add 400 µL H₂O.

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (0.5-mL O2k-chamber): ≥25 µL using a 100 µL Hamilton syringe.
Final concentration: ≥100 mM.

MitoPedia topics: Inhibitor

@@ Line 1: / Line 1: @@
+{{MitoPedia
+|abbr=Azd
+|description='''Sodium azide''' is an inhibitor of [[Complex IV]]/cytochrome ''c'' oxidase (CIV, COX, CcO).
+|info=[[MiPNet03.02]]
+|type=Inhibitor ETS
+}}
+__TOC__
+== Application in [[HRR]] ==
+{{Chemical_description
+|abbr=Azd
+|trivial name=Azide
+|complete name=Sodium azide
+|chem formula=NaN<sub>3</sub>
+|molar mass=65.01
+|vendor=Sigma-Aldrich
+|product number=S2002
+|store at=RT
+|sensitivity=
+|cas=26628-22-8
+|h statements=H300, H310, H330, H373, H410
+|h info=fatal if swallowed, fatal in contact with skin, fatal if inhaled, may cause damage to organs through prolonged or repeated exposure (brain), very toxic to aquatic life with long-lasting effects
+}}<!--:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en&region=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 g·mol<sup>-1</sup>.-->
+:::: 2021-03: Preparation instructions were updated to take the volume of the solute into account (see [[Volume_of_the_solute| Volume of the solute]]). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
+<!--::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span>-->
+::::'''Preparation of 4 M storage and stock solution''' (1 mL, dissolved in H<sub>2</sub>O)  for '''2-mL O2k-chamber''':
+::::# Weigh 260 mg of sodium azide.
+::::# Dissolve in 900 µL H<sub>2</sub>0.
+::::# Divide into 0.5 mL portions.
+::::# Store at -20 °C.
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''2-mL O2k-chamber'''): ≥50 µL using a 100 µL Hamilton syringe.
+::::* Final concentration: ≥100 mM.
+::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)  for '''0.5-mL O2k-chamber''':
+::::# Take 400 µL of the 4 M stock solution.
+::::# Add 400 µL H<sub>2</sub>O.
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''0.5-mL O2k-chamber'''): ≥25 µL using a 100 µL Hamilton syringe.
+::::* Final concentration: ≥100 mM.
+{{MitoPedia methods|type=Inhibitor ETS
+}}
+{{MitoPedia topics
+|mitopedia topic=Inhibitor
+|type=Inhibitor ETS
+}}