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| {{MitoPedia
| | #REDIRECT[[Complex IV]] |
| |abbr=AsTm
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| |description='''AsTm''': Electron flow through [[Complex IV]] (cytochrome ''c'' oxidase) is measured in intact mitochondria after inhibiton of CIII by antimycin A, and addition of [[ascorbate]] (As) and the artificial substrate [[TMPD]] (Tm). Ascorbate has to be titrated first. It reduces [[TMPD]], which further reduces cytochrome ''c'', which is the substrate of CIV. Since CIV is a proton pump of the electron transfer system, the single step of CIV-linked respiration can be measured in different coupling states: AsTm(L), AsTm(P), and AsTm(E). Measurement of CIV activity requires uncoupler titrations to eliminate any potential control by the phosphorylation system, and a cytochrome ''c'' test to avoid any limitation by cytochrome ''c'' release.
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| |info=[[Gnaiger 2002 Biochem Soc Trans]]
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| {{MitoPedia methods}}
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| {{MitoPedia topics
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| |mitopedia topic=Respiratory substrate-coupling state
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| == AsTm(L) ==
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| == AsTm(P) ==
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| == AsTm(E) ==
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