Difference between revisions of "Glucose"
From Bioblast
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|info=[[MiPNet15.09. Yeast: HRR Reference Assay]] | |info=[[MiPNet15.09. Yeast: HRR Reference Assay]] | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at RT; FW = 180.16 | :::'''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at RT; FW = 180.16 | ||
'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
:: | ::::# weigh 360.32 mg of Glucose | ||
:: | ::::# dissolve 1 mL H<sub>2</sub>O in 1.5 mL Eppendorf | ||
''' | :::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 20 µL using a 50 µL syringe | ::::* Titration volume: 20 µL using a 50 µL syringe | ||
::* Final conc. in 2 mL O2k-chamber: 20 mM | ::::* Final conc. in 2 mL O2k-chamber: 20 mM | ||
'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
:: | ::::# weigh 79.2 mg of Glucose | ||
:: | ::::# dissolve 0.2 mL H<sub>2</sub>O in 0.5 mL Eppendorf | ||
''' | :::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 5 µL using a 25 µL syringe | ::::* Titration volume: 5 µL using a 25 µL syringe | ||
::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | ::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | ||
::* Titration volume: 10 µL using a 25 µL syringe | ::::* Titration volume: 10 µL using a 25 µL syringe | ||
::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Substrate and metabolite | |||
}} |
Revision as of 09:02, 11 September 2020
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at RT; FW = 180.16
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve 1 mL H2O in 1.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 20 µL using a 50 µL syringe
- Final conc. in 2 mL O2k-chamber: 20 mM
- Preparation of 2.2 M stock solution (dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 0.2 mL H2O in 0.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 µL using a 25 µL syringe
- Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L)
- Titration volume: 10 µL using a 25 µL syringe
- Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L)
MitoPedia topics:
Substrate and metabolite