Difference between revisions of "Glucose"
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__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | |||
|abbr=Glc | |||
|trivial name=Glucose | |||
|complete name=D-(+)-Glucose | |||
|chem formula=C<sub>6</sub>H<sub>12</sub>0<sub>6</sub> | |||
|molar mass=180.16 | |||
|vendor=Sigma-Aldrich | |||
|product number=G8270 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=50-99-7 | |||
|h statements= | |||
|h info= | |||
}}<!--:::'''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at RT; M = 180.16 g·mol<sup>-1</sup>--> | |||
:::: 2021-03: The preparation instructions were updated to take the volume of the solute into account (see: [[Volume_of_the_solute| Volume of the solute]]). | |||
::::'''Preparation of 2 M stock solution''' (1 mL, dissolved in H<sub>2</sub>O): | |||
::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | |||
::::# weigh 360.32 mg of Glucose | ::::# weigh 360.32 mg of Glucose | ||
::::# dissolve | ::::# dissolve in 775 µL H<sub>2</sub>O in 1.5 mL Eppendorf | ||
:::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
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::::'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 2.2 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O): | ||
::::# weigh 79.2 mg of Glucose | ::::# weigh 79.2 mg of Glucose | ||
::::# dissolve | ::::# dissolve 155 µL H<sub>2</sub>O in 0.5 mL Eppendorf | ||
:::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe. | |||
::::* Final concentration: 5.5 mM (1 g/L) | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe. | |||
::::* Final concentration: 11 mM (2 g/L) | |||
<!-- | |||
::::* Titration volume: 5 µL using a 25 µL syringe | ::::* Titration volume: 5 µL using a 25 µL syringe | ||
::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | ::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | ||
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::::* Titration volume: 10 µL using a 25 µL syringe | ::::* Titration volume: 10 µL using a 25 µL syringe | ||
::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ||
--> | |||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods}} | {{MitoPedia methods}} |
Latest revision as of 12:11, 15 June 2021
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose; C6H1206), Sigma-Aldrich: G8270, store at RT, CAS: 50-99-7, M = 180.16 g·mol-1
- 2021-03: The preparation instructions were updated to take the volume of the solute into account (see: Volume of the solute).
- Preparation of 2 M stock solution (1 mL, dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve in 775 µL H2O in 1.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 20 mM
- Preparation of 2.2 M stock solution (200 µL, dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 155 µL H2O in 0.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 5.5 mM (1 g/L)
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
- Final concentration: 11 mM (2 g/L)
MitoPedia topics:
Substrate and metabolite