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Difference between revisions of "Glucose"

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|abbr=Glc
|abbr=Glc
|description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
|description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
|info=[http://www.oroboros.at/index.php?id=yeast_hrr-reference-assay MiPNet15.09]
|info=[[MiPNet15.09. Yeast: HRR Reference Assay]]
}}
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
}}
}}


== Application in [[HRR]]  ==
__TOC__
== Application in [[HRR]] ==
{{Chemical_description
|abbr=Glc
|trivial name=Glucose
|complete name=D-(+)-Glucose
|chem formula=C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>
|molar mass=180.16
|vendor=Sigma-Aldrich
|product number=G8270
|store at=RT
|sensitivity=
|cas=50-99-7
|h statements=
|h info=
}}<!--:::'''Glc: Glucose''' (D-(+)-Glucose,  C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>);  Sigma G 8270, 100 g, store at RT; M = 180.16 g·mol<sup>-1</sup>-->
:::: 2021-03: The preparation instructions were updated to take the volume of the solute into account (see: [[Volume_of_the_solute| Volume of the solute]]).
 
 
::::'''Preparation of 2 M stock solution''' (1 mL, dissolved in H<sub>2</sub>O):
 
::::# weigh 360.32 mg of Glucose
::::# dissolve in 775 µL H<sub>2</sub>O in 1.5 mL Eppendorf
 
:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]


'''Glc: Glucose''' (D-(+)-Glucose,  C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>);  Sigma G 8270, 100 g, store at R.T.; FW = 180.16
::::* Titration volume ('''2-mL O2k-chamber'''): 20 µL using a 50 µL Hamilton syringe.
::::* Titration volume ('''0.5-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
::::* Final concentration: 20 mM




'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):  
::::'''Preparation of 2.2 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O):  


::1) weigh 360.32 mg of Glucose  
::::# weigh 79.2 mg of Glucose  
::2) dissolve in 1 ml H<sub>2</sub>O
::::# dissolve 155 µL H<sub>2</sub>O in 0.5 mL Eppendorf


:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
::::* Final concentration: 5.5 mM (1 g/L)


'''Oxygraph-2k Titration:'''
::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe.
::::* Final concentration: 11 mM (2 g/L)
<!--
::::* Titration volume: 5 µL using a 25 µL syringe
::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L)


::* Titration volume: 20 µl using a 50 µl syringe
::::* Titration volume: 10 µL using a 25 µL syringe
::* Final conc. in 2 ml O2k-chamber: 20 mM
::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L)
-->
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
}}

Latest revision as of 12:11, 15 June 2021


high-resolution terminology - matching measurements at high-resolution


Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay


Application in HRR

Glc: Glucose (D-(+)-Glucose; C6H1206), Sigma-Aldrich: G8270, store at RT, CAS: 50-99-7, M = 180.16 g·mol-1
2021-03: The preparation instructions were updated to take the volume of the solute into account (see: Volume of the solute).


Preparation of 2 M stock solution (1 mL, dissolved in H2O):
  1. weigh 360.32 mg of Glucose
  2. dissolve in 775 µL H2O in 1.5 mL Eppendorf
»O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
  • Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
  • Final concentration: 20 mM


Preparation of 2.2 M stock solution (200 µL, dissolved in H2O):
  1. weigh 79.2 mg of Glucose
  2. dissolve 155 µL H2O in 0.5 mL Eppendorf
»O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
  • Final concentration: 5.5 mM (1 g/L)
  • Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
  • Final concentration: 11 mM (2 g/L)






MitoPedia topics: Substrate and metabolite