Hasli 2015 Master Thesis

Hasli PR (2015) Characterization of mitochondrial respiration and quality differences in diploid and triploid Atlantic salmon (Salmo salar L.) at 5°C, 10°C and 15°C. Master Thesis 1-147.

Hasli PR (2015) Master Thesis

Abstract: My motivation and reason for this work with my master thesis is due to Norwegians salmon industry perhaps the most cited license distribution through all time: “The green A licensing” in Troms and innmark, effectuated of Erna Solberg (H) first government by the Minister of fisheries Elisabeth Aspaker (H). 60 % of all licenses (12 of 20) are based on use of triploid salmon. This type of salmon is also referred to sterile salmon. Atlantic salmon ±2.2 pounds with diploid and triploid status, and three different temperature regime (5°C, 10°C and 15°C) were in six different wats for two months. It was localized at hall of Environment, Institute for Marine Research, Matre in the rural district Masfjorden (60°52′31″N 5°35′2″Ø). In August 2014, the fish were slaughtered and analysed. The thesis has focused on the comparison of diploid and triploid salmon with several quality parameters. In addition to this, there has been performed characterization of mitochondrial respiration at diploid and triploid salmon before and after smoltification on red muscle, as well as the heart. To analyse the mitochondria tissue homogenization with Shredder PSI-HRR for preparation of sample was used and tested against Biops with/Saponin, which dissolves cell membranes making mitochondria available for analysis. There are ploidy differences in rigor-mortis precursor. Triploid salmon has significantly softer (<0.001) rigor compared with diploid salmon at the three analyzed temperatures. The quality analyses showed that diploid and triploid salmon have no clear quality differences. The analyses show nominal higher drip loss of triploid salmon. The fish filet with the highest level of muscle contraction was the filet with highest amount of drip loss independent of ploidy. Collagenase activity is significantly higher (<0.05) in triploid salmon compared with diploid salmon. Triploid salmon has a higher fat deponation than diploid salmon. The amount of the carotenoid astaxanthin is equal in the analysed fish. HunterLab verify equal colour image at diploid and triploid salmon. Mitochondrial respiration of salmon is not directly affected by plowedity. The mitochondria is well coupled in salmon. The mitochondria was not uncouple with FCCP, and of this reason FCCP does not work as an uncoupler of mitochondria in salmon. Respiratorical leftover oxygen in the heart at triploid salmon in seawater was significantly higher (<0.05) than that of diploid salmon. The reason for this can possibly be the difference in the fat acid composition at diploid and triploid salmon. It was a significantly higher (<0.05) ADP-response in freshwater phase of triploid salmon compared to diploid salmon. This may affect growth rate in freshwater. Shredder gave higher oxygen response in the same tissue volume (<0.001) than biops with/saponin in the oxyograph. The measuring of proteins in the oxyograph is to be done by taking out all the tissue from the chamber, and after that dissolve it. Most probably, it was taken out just a small part of the solution in the chamber. The mitochondrial results are based on weighed wet weight and succinate response.

• O2k-Network Lab: NO As Egelandsdal B

Labels: MiParea: Respiration, mt-Membrane 

Stress:Temperature 

Preparation: Permeabilized tissue, Homogenate 

Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, Theory 

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