Kuznetsov 1998 BTK: Difference between revisions
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|title=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, PΓ₯hlman I-L, Gustafsson L, eds) Chalmers Reproservice, GΓΆteborg:273-6. | |title=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, PΓ₯hlman I-L, Gustafsson L, eds) Chalmers Reproservice, GΓΆteborg:273-6. | ||
|info=[http://www.oroboros.at/fileadmin/user_upload/1998_Kuznetsov-Gnaiger_BTK.pdf pdf: BTK1998: 273-276] | |info=[http://www.oroboros.at/fileadmin/user_upload/1998_Kuznetsov-Gnaiger_BTK.pdf pdf: BTK1998: 273-276] | ||
|authors=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger | |authors=Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger Erich | ||
|year=1998 | |year=1998 | ||
|journal=Chalmers Reproservice | |journal=Chalmers Reproservice | ||
|abstract=Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca<sup>2+</sup>, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria. | |abstract=Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca<sup>2+</sup>, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria. | ||
|mipnetlab= | |mipnetlab=AT Innsbruck Gnaiger E | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
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|organism=Rat | |organism=Rat | ||
|tissues=Heart, Skeletal muscle | |tissues=Heart, Skeletal muscle | ||
|preparations=Permeabilized tissue, Isolated | |preparations=Permeabilized tissue, Isolated mitochondria | ||
|topics=ADP, Oxygen kinetics | |||
|topics=ADP, | |||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
| | |pathways=N, S | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} |
Latest revision as of 04:27, 23 November 2021
Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, PΓ₯hlman I-L, Gustafsson L, eds) Chalmers Reproservice, GΓΆteborg:273-6. |
Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger Erich (1998) Chalmers Reproservice
Abstract: Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca2+, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria.
β’ O2k-Network Lab: AT Innsbruck Gnaiger E
Labels: MiParea: Respiration, Instruments;methods
Organism: Rat
Tissue;cell: Heart, Skeletal muscle
Preparation: Permeabilized tissue, Isolated mitochondria
Regulation: ADP, Oxygen kinetics Coupling state: LEAK, OXPHOS Pathway: N, S HRR: Oxygraph-2k