Measuring hydrogen peroxide
Two ways to measure H2O2 shall be discussed here:
Electrochemical Measurement
For electrochemically (H2O2 electrode) measuring H2O2 (and all other amperometric methods) please consult our"NO" or "amperometric" manual. All stated there about measuring NO is technically also correct for using a H2O2 electrode together with the oxygraph. However, its is sometimes stated that the available H2O2 electrodes may not have the required sensitivity for most ROS studies (WPI states a lower detection limit of "<10 nM" for their electrode). In this manual you will also find which accessories you need to use a H2O2 electrode together with the oxygraph (there are different requirements for different generations of O2ks). The electrode itself has to be bought from a third party.
Fluorescence
The more accepted way here is fluorescence spectroscopy, using Amplex Red (or one of its brand names). The oxygraph has been coupled to full fluorescence spectrophotometers for this purpose Anderson 2010 AJP but also quite "simple" solutions of a LED light source and a photodiode + filter have been attached to the oxygraph for the same purpose. In the second example the potentiometric channel of the O2k multisensor extension was used to directly record the signal in DatLab. In any way, this currently involves a good degree of tinkering by the user, though we will start to develop a commercial solution.
