Meyer 2014 Biochimie

Meyer A, Charles AL, Zoll J, Guillot M, Lejay A, Singh F, Schlagowski AI, Isner-Horobeti ME, Pistea C, Charloux A, Geny B (2014) Cryopreservation with dimethyl sulfoxide prevents accurate analysis of skinned skeletal muscle fibers mitochondrial respiration. Biochimie 100:227-33.

» PMID: 24472439

Meyer A, Charles AL, Zoll J, Guillot M, Lejay A, Singh F, Schlagowski AI, Isner-Horobeti ME, Pistea C, Charloux A, Geny B (2014) Biochimie

Abstract: Impact of cryopreservation protocols on skeletal muscle mitochondrial respiration remains controversial. We showed that oxygen consumption with main mitochondrial substrates in rat skeletal muscles was higher in fresh samples than in cryopreserved samples and that this difference was not fixed but grow significantly with respiration rates with wide fluctuations around the mean difference. Very close results were observed whatever the muscle type and the substrate used. Importantly, the deleterious effects of ischemia-reperfusion observed on fresh samples vanished when cryopreserved samples were studied. These data demonstrate that this technic should probably be performed only extemporaneously. • Keywords: Muscle, Mitochondria, Oxygraphy, Cryopreservation, DMSO, Ischemia

Labels: MiParea: Respiration 

Stress:Cryopreservation, Ischemia-reperfusion  Organism: Rat  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS  Pathway: N, S, CIV