- high-resolution terminology - matching measurements at high-resolution
SUIT-016
Description
Abbreviation: FNS(Oct,GM)
Reference: A Gnaiger 2015 Scand J Med Sci Sports
MitoPedia concepts:
SUIT protocol,
SUIT B
- SUIT-category: FNS(Oct,GM)
- SUIT protocol pattern: diametral
References
| Year | Reference | Organism | Tissue;cell | |
|---|---|---|---|---|
| Gnaiger 2015 Scand J Med Sci Sports | 2015 | Gnaiger E, Boushel R, Søndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612 | Human | Skeletal muscle |
1OctM;2D;3G;4S;5Rot;6Omy;7U;7c-
1OctM;2D;3G;4S;5Rot;6Omy;7U;7c;8Ama
| Step | Respiratory state | Pathway control | Pathway to Q | Comment |
|---|---|---|---|---|
| 1OctM | OctM(L) | F | CETF | |
| 2D | OctM(P) | F | CETF | OXPHOS coupling efficiency in state F. |
| 3G | GMOct(P) | FN | FAO&CI | F/N ratio or (N-F)/N flux control factor of OXPHOS capacity. |
| 4S | GMSOct(P) | FNS | FAO&CI&II | Additive effect of the NS pathway combination, compared to the sum of N+S pathway fluxes measured separately, in the OXPHOS state and the presence of F. |
| 5Rot | S(P) | S | CII | The P/E ratio is not obtained in the FNS state, with preference given to the next steps for OXPHOS coupling efficiency. A succinate concentration of >10 mM may be required for saturating SP capacity. |
| 6Omy | S(L) | S | CII | OXPHOS coupling efficiency in state S, comparable to state F. |
| 7U | S(E) | S | CII | The complete ET capacity in state S may not be obtained after oligomycin. If apparent P/E>1.0, this is corrected to 1.0. |
| 7c | S(E) | S | CII | If cytochrome c control factors = 0.0, the cytochrome c test conducted at this very late ET-pathway state provides an additional indication of stability of the mt-preparation during the experimental assay. Application of the cytochrome c test earlier in the protocol (SUIT-011: 1GM;2D;2c;3S; ..) is preferable, if cytochrome c control factors > 0.0 may be observed occasionally or regulary. If such cytochrome c effects are not exclusion criteria, then OXPHOS states with all substrate combinations can be compared in the presence of cytochrome c. |
| 8Ama | ROX | ROX | ROX may be lower in substrate states earlier in the SUIT protocol. Therefore, this ROX measurement is frequently taken as a methodological control rather than as the basis of ROX correction of mitochondrial respiration (mt). |
