Sonkar 2013 Abstract MiP2013
| Sonkar VK, Dash D, Kulkarn PP (2013) Platelets, amyloid beta and mitochondrial respiration. Mitochondr Physiol Network 18.08. |
Link:
Sonkar VK, Dash D, Kulkarn PP (2013)
Event: MiP2013
Platelets or thrombocytes are the key players in hemostasis and contain >90% of the circulating amyloid precursor protein, which upon proteolytic cleavage by Ξ²-secretase BACE1 (Ξ²-site APP cleaving enzyme 1) and the presenilin-containing Ξ³-secretase complex produces amyloid beta peptide (AΞ²). AΞ², a major mediator of neuronal death, is also cytotoxic to cerebral endothelial cells, vascular smooth muscle cells and can accumulate in the cerebral blood vessels leading to cerebral amyloid angiopathy. In this report we have evaluated the effect of AΞ² active fragment containing amino acid sequence 25-35 (AΞ²25-35) on platelet activity and mitochondrial respiration. Exogenous AΞ²25-35 was found to induce platelet aggregation, PAC-1binding and P-selectin exposure in washed human platelets, which was comparable to that induced by other physiological agonists. AΞ²25-35 also induced ATP release indicative of secretion from platelet dense granules. High-resolution respirometry (HRR) showed that AΞ²25-35 elicited sharp rise in mitochondrial respiration in intact platelets. Thus, AΞ² induced platelet activation that was associated with an increase in oxygen consumption rate.
β’ O2k-Network Lab: IN Varanasi Dash D
Labels:
Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
Affiliations and author contributions
Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Email: sonkarvijay@gmail.com
- Corresponding author: ddass@satyam.net.in
