TMPD: Difference between revisions
No edit summary |
No edit summary |
||
Line 3: | Line 3: | ||
|description=N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride, C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>.2HCl; applied as an artificial substrate for reducing cytochrome ''c'' in the respirometric assay for cytochrome c oxidase activity; is maintained in a reduced state by ascorbate; undergoes autooxidation as a function of oxygen pressure, ascrobate and cytochrome c concentration. | |description=N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride, C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>.2HCl; applied as an artificial substrate for reducing cytochrome ''c'' in the respirometric assay for cytochrome c oxidase activity; is maintained in a reduced state by ascorbate; undergoes autooxidation as a function of oxygen pressure, ascrobate and cytochrome c concentration. | ||
|info=MiPNet06.06, MiPNet09.12 | |info=MiPNet06.06, MiPNet09.12 | ||
|type=Substrate ETS | |||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Substrate and metabolite | |||
|type=Substrate ETS | |type=Substrate ETS | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|preparations=Oxidase; Biochemical Oxidation, Enzyme | |preparations=Oxidase; Biochemical Oxidation, Enzyme | ||
|enzymes=Complex IV; Cytochrome c Oxidase | |||
|kinetics=Reduced Substrate; Cytochrome c | |kinetics=Reduced Substrate; Cytochrome c | ||
| | |instruments=Chemicals; Media | ||
|type=Substrate ETS | |||
}} | }} |
Revision as of 14:22, 9 March 2011
Description
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride, C10H16N2.2HCl; applied as an artificial substrate for reducing cytochrome c in the respirometric assay for cytochrome c oxidase activity; is maintained in a reduced state by ascorbate; undergoes autooxidation as a function of oxygen pressure, ascrobate and cytochrome c concentration.
Abbreviation: Tm
Reference: MiPNet06.06, MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Labels:
Preparation: Oxidase; Biochemical Oxidation"Oxidase; Biochemical Oxidation" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Enzyme
Enzyme: Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.