Takahashi 2018 Sci Rep

Takahashi K, Miura Y, Ohsawa I, Shirasawa T, Takahashi M (2018) In vitro rejuvenation of brain mitochondria by the inhibition of actin polymerization. Sci Rep 8:15585.

» PMID: 30348987 Open Access

Takahashi K, Miura Y, Ohsawa I, Shirasawa T, Takahashi M (2018) Sci Rep

Abstract: The oxygen consumption rate (OCR) and cytochrome c oxidase (CcO) activity of respiratory complex IV (CIV) in brain mitochondria significantly decline in middle-aged male mice compared to younger male mice. To explore the mechanisms underlying the regulation of brain mitochondrial function, we examined CIV-associated proteins, and identified actin inside the isolated brain mitochondria. Inhibiting actin polymerization using cytochalasin B (CB) significantly enhanced the OCR and CcO activity of CIV in the mitochondria. These changes were accompanied by a significant reduction in the amount of CIV-bound cytochrome c (cyt c). Actin was also associated with respiratory complex III (CIII); however, the amount of CIII-bound cyt c increased significantly after treatment of the mitochondria with CB. In contrast, no significant alteration in the assembly or the CcO activity of CIV in CIV-containing supercomplexes or CIV monomers was induced by CB. These results suggest that mitochondrial actin plays a crucial role in the regulation of the CcO activity and OCR of CIV with modification of the retention of cyt c between CIV and CIII.

• Bioblast editor: Plangger M • O2k-Network Lab: JP Tokyo Tanaka M

Labels: MiParea: Respiration  Pathology: Aging;senescence 

Organism: Mouse  Tissue;cell: Nervous system  Preparation: Isolated mitochondria  Enzyme: Complex III, Complex IV;cytochrome c oxidase 

Coupling state: OXPHOS  Pathway: N  HRR: Oxygraph-2k 

Labels, 2018-11, JP