Talk:PBI-Shredder SG3
From Bioblast
Advantages of the Shredder
- low shear mechanical device for gentle, rapid and safe disruption of tissues
- three position lever for setting reproducible force to the sample during the shredding process
- gentle enough for isolating intact, functional mitochondrial
- powerful enough to rapidly break apart difficult samples
- standardize preparations of high quality
- enables reproducible results
- easy handling, especially for beginners
- processing containers:
- > standard tubes for ambient pressure processing
- > specialized tubes for ultra high pressure processing
- > closed containers help to ensure safety throughout the entire sample preparation process
- > excellent for collection, storage, transport and processing
- high quality of functional mitochondria is obtained from skeletal muscel and kidney as evaluated by high resolution respirometry
Traditional methods for homogenization
- homogenizer-based protocols that require extensive operator experience (highly skilled personnel) to obtain reproducible high-quality preparations
- these methods limit dissemination, impede scale-up, and contribute to difficulities in reproducing experimental results over time and across laboratories
- during manual homogenization it is difficult to know when sufficient tissue disruption has occured - overhomogenization!
- the current protocols rely on Dounce, Potter-Elvehjem, and rotor/stator shearing homogenization
- Pallotti F, Lenaz G (2007) Isolation and Subfractionation of Mitochondria from Animal Cells and Tissue Culture Lines. Methods Cell Biol 80:3-44.
- Frezza C, Cipolat S, Scorrano L (2007) Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nat Protoc 2:287-95.
- Kaufmann P, Török M, Zahno A, Waldhauser KM, Brecht K, Krähenbühl S (2006) Toxicity of statins on rat skeletal muscle mitochondria.Cell Mol Life Sci 63:2415-25.
