Tetraphenylphosphonium: Difference between revisions
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* [[O2k-TPP%2B_and_Ca2%2B_ISE-Module |O2k-TPP+ ISE-Module]] | * [[O2k-TPP%2B_and_Ca2%2B_ISE-Module |O2k-TPP+ ISE-Module]] | ||
'''First TPP publication with HRR''' [[Brown 2011 Am J Physiol Regul Integr Comp Physiol]] | '''First TPP publication with HRR''' [[Brown 2011 Am J Physiol Regul Integr Comp Physiol]] | ||
{{Technical support integrated}} | |||
== TPP<sup>+</sup> inhibitory effect == | |||
A major first task in establishing a system for measurement of [[Mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. This important 'side effect' of TPP+ and TPMP+ (and various dyes) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range [[MiPNet14.05]]. |
Revision as of 13:42, 11 May 2015
Description
Tetraphenylphosphonium (TPP+). A molecular probe in conjunction with an ion selective electrode (ISE) for measuring the mitochondrial membrane potential.
See also: External unspecific binding of TPP+ and Internal unspecific binding of TPP+.
Abbreviation: TPP+
Reference: MiPNet14.05
First TPP publication with HRR Brown 2011 Am J Physiol Regul Integr Comp Physiol
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
TPP+ inhibitory effect
A major first task in establishing a system for measurement of Mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. This important 'side effect' of TPP+ and TPMP+ (and various dyes) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range MiPNet14.05.