Difference between revisions of "Biological contamination"
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:::: Biological contamination of the O2k-Chamber is detected as a high O<sub>2</sub> flux in the closed chamber, | :::: Biological contamination of the O2k-Chamber is detected as a high O<sub>2</sub> flux, ''J''°<sub>O2</sub>, in the [https://wiki.oroboros.at/index.php/Closed_chamber | closed chamber], obtained in an [https://wiki.oroboros.at/index.php/Oxygen_sensor_test | Q1: Oxygen sensor test] or [Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]. | ||
:::: The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber from the O2k and using a strong acid. | :::: The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber from the O2k and using a strong acid. | ||
Revision as of 15:26, 11 August 2020
- high-resolution terminology - matching measurements at high-resolution
Biological contamination
Description
Biological contamination may be caused by microbial growth in the O2k-Chamber or in the experimental medium.
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- Biological contamination of the O2k-Chamber is detected as a high O2 flux, J°O2, in the | closed chamber, obtained in an | Q1: Oxygen sensor test or [Oxygen flux - instrumental background |QC2: Instrumental O2 background test]].
- The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as MiR05. Make sure the 70% ethanol does not contain any additives; e.g., 70 % ethanol used in hospital settings may contain antiseptics (information provided by Garcia-Roves PM). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under Cleaning the glass chamber by removing the glass chamber from the O2k and using a strong acid.
