Coupling-control ratio: Difference between revisions

Revision as of 15:37, 3 June 2020

high-resolution terminology - matching measurements at high-resolution

Coupling-control ratio

Description

Coupling-control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial pathway-control state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, Electron transfer pathway). In living cells, the OXPHOS state cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, J_ref, is defined by taking J_ref as the maximum flux, i.e. flux in the ET state, E, such that the lower and upper limits of the CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for living cells, L/E and R/E.

Abbreviation: CCR

Reference: Flux control ratio

The Blue Book 2014: Fig. 2.4.

Coupling control ratios related to free capacities

L/P coupling control ratio, L/P

Free OXPHOS capacity, ≈P = P-L

OXPHOS-coupling efficiency, j_≈P = ≈P/P = 1-L/P

L/R coupling control ratio, L/R

Free ROUTINE activity, ≈R = R-L

ROUTINE-coupling efficiency, j_≈R = ≈R/R = 1-L/R

LEAK-control ratio, L/E

Free ET capacity, ≈E = E-L

ET-coupling efficiency, j_≈E = ≈E/E = 1-L/E

netOXPHOS-control ratio, ≈P/E=(P-L)/E

Free OXPHOS capacity, ≈P = P-L

netROUTINE-control ratio, ≈R/E=(R-L)/E

Free ROUTINE activity, ≈R = R-L

More details » ET-coupling efficiency

Coupling control ratios related to excess capacities

OXPHOS-control ratio, P/E

Excess E-P capacity, ExP = E-P

Excess E-P capacity factor, j_ExP = ExP/E = 1-P/E

ROUTINE-control ratio, R/E

Excess E-R capacity, ExR = E-R

Excess E-R capacity factor, j_ExR = ExR/E = 1-R/E

Compare

» Coupling control factor, CCF = 1-CCR

» Respiratory acceptor control ratio, RCR

References

Bioblast link	Reference	Year
Gnaiger 2020 BEC MitoPathways	Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002	2020
BEC 2020.1 doi10.26124bec2020-0001.v1	Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1	2020

Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Rate

» Normalization of rate

» Flow

» Oxygen flow

» Flux

» Oxygen flux

» Flux control ratio

» Coupling-control ratio

» Pathway control ratio

» Flux control efficiency

Quantities for normalization

» Count in contrast to Number

» Mitochondrial marker

» O2k-Protocols: mitochondrial and marker-enzymes

» Citrate synthase activity

General

» Extensive quantity

» Specific quantity

» Advancement

» Motive unit

» Iconic symbols

Related keyword lists

» Keywords: Concentration and pressure

MitoPedia concepts: Respiratory control ratio

MitoPedia methods: Respirometry

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=''CCR''
-|description='''Coupling-control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[pathway-control state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[Electron transfer pathway]]). In living cells, the OXPHOS state cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ET state, ''E'', such that the lower and upper limits of the ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for living cells, [[L/E|''L/E'']] and [[R/E control ratio|''R/E'']].
+|description='''Coupling-control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[pathway-control state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[Electron transfer pathway]]). In living cells, the OXPHOS state cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ET state, ''E'', such that the lower and upper limits of the ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for living cells, [[L/E|''L/E'']] and [[ROUTINE-control ratio|''R/E'']].
 |info=[[Flux control ratio]]
 }}