Fluorometric dyes: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|description=[[ | |description=[[Extrinsic fluorophores]]; fluorescent markers. | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method= | |mitopedia method=Fluorometry | ||
}} | }} | ||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
== | == Fluorometric dyes for direct quantification == | ||
*ROS (H<sub>2</sub>O<sub>2</sub>): [[Amplex red]] | *ROS (H<sub>2</sub>O<sub>2</sub>): [[Amplex red]] | ||
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*ROS (HOO-.): [[MitoSOX]] | *ROS (HOO-.): [[MitoSOX]] | ||
== | == Fluorometric dyes for ratiometric excitation == | ||
*Ca2+: [[Fura2]] | *Ca2+: [[Fura2]] | ||
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*Redox state: [[roGFP]] | *Redox state: [[roGFP]] | ||
== | == Fluorometric dyes for ratiometric emmission == | ||
*Ca2+: [[Indo1]] | *Ca2+: [[Indo1]] | ||
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*[[NADH]] | *[[NADH]] | ||
Latest revision as of 14:28, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
Fluorometric dyes
Description
Extrinsic fluorophores; fluorescent markers.
MitoPedia methods: Fluorometry
Fluorometric dyes for direct quantification
- ROS (H2O2): Amplex red
- Ca2+ - Dye: Fura2
- Ca2+ - Dye: Indo1
- Ca2+ - Dye: Calcium green
- mt-Membrane potential: Safranin
- mt-Membrane potential: TMRM
- mt-Membrane potential, H2O2: Rhodamin 123
- pH: Fluorescin
- pH: HPTS
- Mg2+: Magnesium organge, Magnesium green
- ROS (HOO-.): MitoSOX
