Difference between revisions of "MiPNet16.04 IOC62"
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{{ | {{OROBOROS header page name}} | ||
{{Publication | {{Publication | ||
|title=[[Image:OROBOROS-solo.png|right|50px|link=http://www.oroboros.at| | |title=[[Image:OROBOROS-solo.png|right|50px|link=http://www.oroboros.at|Oroboros Instruments]] | ||
'''Innsbruck AT''', 2011 Jul 11-13. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, '''IOC62'''. | |||
|authors= | |authors=Oroboros | ||
|year=2011-07-11 | |year=2011-07-11 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]] | |abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]] | ||
'''62th | '''62th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry'''. Innsbruck, AT; 2011 July 11-13. | ||
|mipnetlab=AT Innsbruck Oroboros | |||
|mipnetlab= AT Innsbruck | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=ORO, IOC, 2011 | |additional=ORO, IOC, 2011 | ||
}} | }} | ||
__TOC__ | __TOC__ | ||
== Lecturer == | == Lecturer == | ||
[[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]] | [[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]] | ||
[[Gnaiger Erich]], CEO, | [[Gnaiger Erich]], CEO, Oroboros Instruments | ||
== General information == | |||
The July-Workshop includes a basic introduction to the [[Oroboros O2k]], overview presentations on high-resolution respirometry ([[HRR]]), and applications in mitochondrial physiology. Specific emphasis is placed on hands-on practical sessions with the instrument and the software DatLab 4.3, to introduce the participants into the technical and operational details of the method. An overview is given on the [[O2k-MultiSensor]] System, using our TPP+ electrode for simultaneous measurement of mt-membrane potential and oxygen consumption. | |||
As an outlook to INNOVATION, we will present our newest perspectives on the O2k-Fluorometer, which is being developed in the frame of the project MitoCom Tyrol. It is intended not only to develop a new generation instrument, but also to maintain our strategy of options for modular upgrading the current O2k-Models. The workshop programme is complemented by lectures and experiments based on substrate-uncoupler-inhibitor titration ([[SUIT]]) protocols. Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols. | |||
== Programme == | |||
'''Monday, 11. July''' | |||
* 09:00-12:30 Introduction to High-Resolution Respirometry | |||
* 14:00-17:30 Hands-on experiments with the O2k: Calibration and instrumental background oxygen flux | |||
'''Tuesday, 12. July''' | |||
* 09:00-12:30 The O2k-Fluorimeter in development: Demo experiment with tissue homogenate | |||
* 14:00-17:30 Hands-on experiments with the O2k: TPP+ calibration and application with tissue homogenate | |||
'''Wednesday, 13. July''' | |||
* 09:00-12:30 O2k-MultiSensor High-Resolution Respirometry and data analysis: DatLab and application of Excel files. From TPP+ concentration to mitochondrial membrane potential. | |||
* 14:00-17:30 Mitochondrial respiratory physiology: Protocols with substrate-uncoupler-inhibitor titrations for coupling and substrate control. | |||
== Recommended reading == | == Recommended reading == | ||
| Line 28: | Line 60: | ||
=== SUIT protocols for high-resolution respirometry === | === SUIT protocols for high-resolution respirometry === | ||
* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small | * Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]] | ||
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327- | * Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast Access]] | ||
=== HRR and O2k-Fluorometry === | === HRR and O2k-Fluorometry === | ||
* »[[MiPNet17.05 O2k- | * »[[MiPNet17.05 O2k-Fluo LED2-Module |Manual: O2k-Fluo LED2-Module]] | ||
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]] | * Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]] | ||
* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | * »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | ||
=== Mitochondrial pathways === | === Mitochondrial pathways === | ||
* Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. | * Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck: 64 pp. [[Gnaiger 2012 MitoPathways |»Open Access]] - '''''handout to O2k-Workshop participants'''''. | ||
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] | [[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] | ||
O2k-Workshops are listed as [[MitoGlobal Events]]. | O2k-Workshops are listed as [[MitoGlobal Events]]. | ||
* This workshop is a component of the K-Regio project ''[[ | * This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''. | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] | ||
Latest revision as of 02:27, 9 October 2019
MiPNet16.04 IOC62
 Innsbruck AT, 2011 Jul 11-13. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC62. |
Oroboros (2011-07-11) Mitochondr Physiol Network
Abstract:
62th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry. Innsbruck, AT; 2011 July 11-13.
• O2k-Network Lab: AT Innsbruck Oroboros
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2011
Lecturer
Gnaiger Erich, CEO, Oroboros Instruments
General information
The July-Workshop includes a basic introduction to the Oroboros O2k, overview presentations on high-resolution respirometry (HRR), and applications in mitochondrial physiology. Specific emphasis is placed on hands-on practical sessions with the instrument and the software DatLab 4.3, to introduce the participants into the technical and operational details of the method. An overview is given on the O2k-MultiSensor System, using our TPP+ electrode for simultaneous measurement of mt-membrane potential and oxygen consumption.
As an outlook to INNOVATION, we will present our newest perspectives on the O2k-Fluorometer, which is being developed in the frame of the project MitoCom Tyrol. It is intended not only to develop a new generation instrument, but also to maintain our strategy of options for modular upgrading the current O2k-Models. The workshop programme is complemented by lectures and experiments based on substrate-uncoupler-inhibitor titration (SUIT) protocols. Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.
Programme
Monday, 11. July
- 09:00-12:30 Introduction to High-Resolution Respirometry
- 14:00-17:30 Hands-on experiments with the O2k: Calibration and instrumental background oxygen flux
Tuesday, 12. July
- 09:00-12:30 The O2k-Fluorimeter in development: Demo experiment with tissue homogenate
- 14:00-17:30 Hands-on experiments with the O2k: TPP+ calibration and application with tissue homogenate
Wednesday, 13. July
- 09:00-12:30 O2k-MultiSensor High-Resolution Respirometry and data analysis: DatLab and application of Excel files. From TPP+ concentration to mitochondrial membrane potential.
- 14:00-17:30 Mitochondrial respiratory physiology: Protocols with substrate-uncoupler-inhibitor titrations for coupling and substrate control.
Recommended reading
O2k-Core Manual
- New: »O2k-Core Manual.pdf
SUIT protocols for high-resolution respirometry
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
HRR and O2k-Fluorometry
- »Manual: O2k-Fluo LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »Open Access
- »O2k-Fluorometry
Mitochondrial pathways
- Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.
