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Difference between revisions of "MiPNet22.03 IOC119 Innsbruck AT"

From Bioblast
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__TOC__
== Programme ==
{| class='wikitable'
|-
|style="width: 10em"|'''Tuesday 28th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|17:00 - 18:00
|Welcome - common aims
|MiP''Art''
|-
|}
{| class='wikitable'
|-
|style="width: 10em"|'''Wednesday 1st'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Mitochondrial and cell research
|MiP''Art''
|-
|10:00
|OroboPOS service and O2k instrumental setup - overview with videoclips
|MiP''Art''
|-
|10:30
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|MiP''Art''
|-
|12:00
|''Lunch break''
|
|-
|13:00
|Instrumental quality control 1: oxygen calibration
|MiP''Art''
|-
|14:00
|Instrumental quality control 2: Instrumental O2 background
|MiP''Art''
|-
|15:00
|''Coffee''
|
|-
|15:30
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|MitoFit ORO Lab
|-
|14:30-18:00
|Hands-on: Instrumental quality control 1 and 2.
|MitoFit ORO Lab - MiP''Art''
|-
|}
{| class='wikitable'
|-
|style="width: 10em"|'''Thursday 2nd'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Demo: Instrumental quality control 1. Introduction to SUIT protocols RP1&RP2
|MiP''Art''
|-
|10:00
|''Coffee''
|
|-
|10:30
|O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
|MiP''Art''
|-
|12:00
|''Lunch break''
|
|-
|13:00
|Introduction to DatLab analysis
|MiP''Art''
|-
|15:00
|''Coffee''
|
|-
|15:30-18:00
|Individual DatLab analysis + summary
|MiP''Art''
|-
|}
{| class='wikitable'
|-
|style="width: 10em"|'''Friday 3rd'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|SUIT protocols
|MiP''Art''
|-
|10:30
|''Coffee''
|
|-
|11:00
|Q&A session on HRR and OXPHOS analysis: Design of experimental protocols
|MiP''Art''
|-
|12:00
|Lunch break
|
|-
|13:00
|O2k-Core and O2k-Multisensor
|MiP''Art''
|-
|14:00
|DatLab analysis
|MiP''Art''
|-
|15:00
|''Coffee''
|
|-
|15:30
|DatLab analysis
|MiP''Art''
|-
|16:15
|The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions
|MiP''Art''
|-
|17:30
|Farewell activity
|MiP''Art''
|-
|}


== General information ==
== General information ==
Line 17: Line 159:
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.


== Programme ==
== Registration ==
::::View the programme [http://www.bioblast.at/images/c/ce/Programme.pdf here]
::::Fully booked.
::::'''Maximum number of 6 labs.'''
::::'''Maximum number of 6 participants.'''


== Tutors ==
== Tutors ==

Revision as of 15:36, 24 February 2017

                

MiPNet22.03 IOC119 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119.


OROBOROS (2017-02-27) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.


• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit


Programme

Tuesday 28th Topic Room
17:00 - 18:00 Welcome - common aims MiPArt
Wednesday 1st Topic Room
09:00 Mitochondrial and cell research MiPArt
10:00 OroboPOS service and O2k instrumental setup - overview with videoclips MiPArt
10:30 Hands-on: OroboPOS service and O2k instrumental setup MiPArt
12:00 Lunch break
13:00 Instrumental quality control 1: oxygen calibration MiPArt
14:00 Instrumental quality control 2: Instrumental O2 background MiPArt
15:00 Coffee
15:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. MitoFit ORO Lab
14:30-18:00 Hands-on: Instrumental quality control 1 and 2. MitoFit ORO Lab - MiPArt
Thursday 2nd Topic Room
09:00 Demo: Instrumental quality control 1. Introduction to SUIT protocols RP1&RP2 MiPArt
10:00 Coffee
10:30 O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. MiPArt
12:00 Lunch break
13:00 Introduction to DatLab analysis MiPArt
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary MiPArt
Friday 3rd Topic Room
09:00 SUIT protocols MiPArt
10:30 Coffee
11:00 Q&A session on HRR and OXPHOS analysis: Design of experimental protocols MiPArt
12:00 Lunch break
13:00 O2k-Core and O2k-Multisensor MiPArt
14:00 DatLab analysis MiPArt
15:00 Coffee
15:30 DatLab analysis MiPArt
16:15 The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions MiPArt
17:30 Farewell activity MiPArt

General information

The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
» Information on travel and venue: IOC Innsbruck.

Registration

Fully booked.
Maximum number of 6 participants.

Tutors



Participants

Participant Institution
Anja Bufe AT Innsbruck OROBOROS: OROBOROS INSTRUMENTS, Innsbruck (AT)
Eugenia Carvalho US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Matthew Cotter US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Ivone Leong SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Yue Hugh Guan
 Yue Hugh Guan CN Shanghai Yi X: East China University of Science and Technology, Shanghai (CN)
Vasco Sequeira Oliveira NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL)
Anna-Maria Wintler SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Evelyn Zacharewicz NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL)

Contact

instruments@oroboros.at


OROBOROS INSTRUMENTS
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


Labels:






ORO, IOC, 2017, MitoFit, Next 

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