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Difference between revisions of "MiPNet22.03 IOC119 Innsbruck AT"

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{{OROBOROS header page name}}  
{{OROBOROS header page name}}  
{{Publication
{{Publication
|title= [[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title= [[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]]
'''Innsbruck AT''', 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic '''IOC119'''.
'''Innsbruck AT''', 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic '''IOC119'''.
|info=
|info=
|authors=OROBOROS
|authors=Oroboros
|year=2017-02-27
|year=2017-02-27
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2017 Feb 27-Mar 02.
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2017 Feb 28-Mar 03.
|mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit
|mipnetlab=AT_Innsbruck_Oroboros , AT_Innsbruck_MitoFit
}}
{{Labeling
|additional=ORO, IOC, 2017, MitoFit, Next
}}
}}


[[File:IOC119 Innsbruck.JPG|right|400px|Participants of IOC119 and the Oroboros tutors]]
__TOC__






__TOC__


== Programme ==
== Programme ==
The programme will be along the lines of [[IOC114]].
::::{| class='wikitable'
|-
|style="width: 10em"|'''Tuesday 28th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|17:00 - 18:00
|Welcome - common aims
|MiP''Art''
|-
|}
 
::::{| class='wikitable'
|-
|style="width: 10em"|'''Wednesday 1st'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Mitochondrial and cell research
|MiP''Art''
|-
|10:00
|OroboPOS service and O2k instrumental setup - overview with videoclips
|MiP''Art''
|-
|10:30
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|MiP''Art''
|-
|12:00
|''Lunch break''
|
|-
|13:00
|Instrumental quality control 1: oxygen calibration
|MiP''Art''
|-
|14:00
|Instrumental quality control 2: Instrumental O2 background
|MiP''Art''
|-
|15:00
|''Coffee''
|
|-
|15:30-18:00
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|ORO-MitoFit Lab
|-
|}
 
::::{| class='wikitable'
|-
|style="width: 10em"|'''Thursday 2nd'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Introduction to SUIT protocols RP1&RP2
|MiP''Art''
|-
|10:00
|''Coffee''
|
|-
|10:30
|O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
|MiP''Art''
|-
|12:00
|''Lunch break''
|
|-
|13:00
|Introduction to DatLab analysis
|MiP''Art''
|-
|15:00
|''Coffee''
|
|-
|15:30-18:00
|Individual DatLab analysis + summary
|ORO-MitoFit Lab
|-
|}
 
::::{| class='wikitable'
|-
|style="width: 10em"|'''Friday 3rd'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|SUIT protocols
|MiP''Art''
|-
|10:30
|''Coffee''
|
|-
|11:00
|Q&A session on HRR and OXPHOS analysis: Design of experimental protocols
|MiP''Art''
|-
|12:00
|Lunch break
|
|-
|13:00
|O2k-Core and O2k-Multisensor
|MiP''Art''
|-
|14:00
|DatLab analysis
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30
|DatLab analysis
|ORO-MitoFit Lab
|-
|16:15
|The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions
|MiP''Art''
|-
|17:30
|Farewell activity
|MiP''Art''
|-
|}
 
== General information ==
::::The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
[[File:TRACT logo.png|right|120px|TRACT]]
::::Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR).
 
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
 
== Registration ==
::::Fully booked.
::::'''Maximum number of 6 participants.'''
 
== Tutors ==
<br/>
<gallery mode=default perrow=6 widths="140px" heights="150px">
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Gnaiger Erich]]'''
File:SumbalovaZ.JPG|'''[[Sumbalova Z|Sumbalova Zuzana]]'''
File:NevesP.JPG|'''[[Neves P|Neves Pedro]]'''
File:PassruggerM.JPG|'''[[Passrugger M|Passrugger Manuela]]'''
File:GarciaL.JPG|'''[[Garcia-Souza LF|Garcia e Souza Luiz Felipe]]'''
File:Velika B.jpg|'''[[Cizmarova B|Cizmarova Beata]]'''
</gallery>
 
 
== Participants ==
 
::::{| class="wikitable"
|-
!
! Participant
! Institution
|-
|
| [[Bufe A| Anja Bufe]]
| '''[[AT Innsbruck Oroboros ]]:''' Oroboros Instruments, Innsbruck (AT)
|-
|
| [[Carvalho E| Eugenia Carvalho]]
| '''[[US AR Little Rock Borsheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
|-
|
| [[Cotter M| Matthew Cotter]]
| '''[[US AR Little Rock Borsheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
|-
|
| [[Leong I| Ivone Leong]]
| '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE)
|-
|
| [[Guan YH| Yue Hugh Guan]]
| Shanghai (CN)
|-
|
| [[Sequeira Oliveira V| Vasco Sequeira Oliveira]]
| '''[[NL Amsterdam Wuest RC]]:''' VU University Medical Center, Amsterdam (NL)
|-
|
| [[Wintler AM|Anna-Maria Wintler]]
| '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE)
|-
|
| [[Zacharewicz E| Evelyn Zacharewicz]]
| '''[[NL Maastricht Schrauwen P]]:''' Department of Human Biology and Movement Sciences, Maastricht University (NL)
|-
|}


== Contact ==
== Contact ==
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:::: OROBOROS INSTRUMENTS
:::: Oroboros Instruments
:::: high-resolution respirometry  
:::: high-resolution respirometry  
:::: Schöpfstrasse 18
:::: Schöpfstrasse 18
Line 39: Line 236:
== Project support==
== Project support==
:::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
:::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
:::: [[File:Sponsors.png| 750 px]]
:::: [[File:Sponsors.png| 750 px]] [[File:TRACT logo.png|120px|TRACT]]


== IOC recommended reading ==
== IOC recommended reading ==
::::» '''[[IOC recommended reading]]'''
::::» '''[[IOC recommended reading]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]




Line 51: Line 248:
  O2k-Workshops and MitoFit Coaching Days are listed as [[MitoGlobal Events]].
  O2k-Workshops and MitoFit Coaching Days are listed as [[MitoGlobal Events]].


{{Labeling
|instruments=Oxygraph-2k
|additional=ORO, IOC, MitoFit, TRACT, 2017
}}


[[Category:O2k-Workshops]]
[[Category:O2k-Workshops]]

Latest revision as of 10:56, 31 March 2021

                

MiPNet22.03 IOC119 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119.


Oroboros (2017-02-27) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.


• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


Participants of IOC119 and the Oroboros tutors



Programme

Tuesday 28th Topic Room
17:00 - 18:00 Welcome - common aims MiPArt
Wednesday 1st Topic Room
09:00 Mitochondrial and cell research MiPArt
10:00 OroboPOS service and O2k instrumental setup - overview with videoclips MiPArt
10:30 Hands-on: OroboPOS service and O2k instrumental setup MiPArt
12:00 Lunch break
13:00 Instrumental quality control 1: oxygen calibration MiPArt
14:00 Instrumental quality control 2: Instrumental O2 background MiPArt
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 2nd Topic Room
09:00 Introduction to SUIT protocols RP1&RP2 MiPArt
10:00 Coffee
10:30 O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. MiPArt
12:00 Lunch break
13:00 Introduction to DatLab analysis MiPArt
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 3rd Topic Room
09:00 SUIT protocols MiPArt
10:30 Coffee
11:00 Q&A session on HRR and OXPHOS analysis: Design of experimental protocols MiPArt
12:00 Lunch break
13:00 O2k-Core and O2k-Multisensor MiPArt
14:00 DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30 DatLab analysis ORO-MitoFit Lab
16:15 The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions MiPArt
17:30 Farewell activity MiPArt

General information

The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
TRACT
Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR).
» Information on travel and venue: IOC Innsbruck.

Registration

Fully booked.
Maximum number of 6 participants.

Tutors



Participants

Participant Institution
Anja Bufe AT Innsbruck Oroboros : Oroboros Instruments, Innsbruck (AT)
Eugenia Carvalho US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Matthew Cotter US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Ivone Leong SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Yue Hugh Guan Shanghai (CN)
Vasco Sequeira Oliveira NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL)
Anna-Maria Wintler SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Evelyn Zacharewicz NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL)

Contact

instruments@oroboros.at


Oroboros Instruments
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png TRACT

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


Labels:





HRR: Oxygraph-2k 

ORO, IOC, MitoFit, TRACT, 2017 

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