MiPNet22.03 IOC119 Innsbruck AT: Difference between revisions
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{{OROBOROS header page name}} | {{OROBOROS header page name}} | ||
{{Publication | {{Publication | ||
|title= [[Image:OROBOROS-solo.png|right|60px|link= | |title= [[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]] | ||
'''Innsbruck AT''', 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic '''IOC119'''. | '''Innsbruck AT''', 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic '''IOC119'''. | ||
|info= | |info= | ||
|authors= | |authors=Oroboros | ||
|year=2017-02-27 | |year=2017-02-27 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | ||
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2017 Feb 28-Mar 03. | '''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2017 Feb 28-Mar 03. | ||
|mipnetlab= | |mipnetlab=AT_Innsbruck_Oroboros , AT_Innsbruck_MitoFit | ||
}} | }} | ||
[[File:IOC119 Innsbruck.JPG|right|400px|Participants of IOC119 and the Oroboros tutors]] | |||
__TOC__ | __TOC__ | ||
== Programme == | |||
::::{| class='wikitable' | |||
|- | |||
|style="width: 10em"|'''Tuesday 28th''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Room''' | |||
|- | |||
|17:00 - 18:00 | |||
|Welcome - common aims | |||
|MiP''Art'' | |||
|- | |||
|} | |||
::::{| class='wikitable' | |||
|- | |||
|style="width: 10em"|'''Wednesday 1st''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Room''' | |||
|- | |||
|09:00 | |||
|Mitochondrial and cell research | |||
|MiP''Art'' | |||
|- | |||
|10:00 | |||
|OroboPOS service and O2k instrumental setup - overview with videoclips | |||
|MiP''Art'' | |||
|- | |||
|10:30 | |||
|'''Hands-on''': OroboPOS service and O2k instrumental setup | |||
|MiP''Art'' | |||
|- | |||
|12:00 | |||
|''Lunch break'' | |||
| | |||
|- | |||
|13:00 | |||
|Instrumental quality control 1: oxygen calibration | |||
|MiP''Art'' | |||
|- | |||
|14:00 | |||
|Instrumental quality control 2: Instrumental O2 background | |||
|MiP''Art'' | |||
|- | |||
|15:00 | |||
|''Coffee'' | |||
| | |||
|- | |||
|15:30-18:00 | |||
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis. | |||
|ORO-MitoFit Lab | |||
|- | |||
|} | |||
::::{| class='wikitable' | |||
|- | |||
|style="width: 10em"|'''Thursday 2nd''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Room''' | |||
|- | |||
|09:00 | |||
|Introduction to SUIT protocols RP1&RP2 | |||
|MiP''Art'' | |||
|- | |||
|10:00 | |||
|''Coffee'' | |||
| | |||
|- | |||
|10:30 | |||
|O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. | |||
|MiP''Art'' | |||
|- | |||
|12:00 | |||
|''Lunch break'' | |||
| | |||
|- | |||
|13:00 | |||
|Introduction to DatLab analysis | |||
|MiP''Art'' | |||
|- | |||
|15:00 | |||
|''Coffee'' | |||
| | |||
|- | |||
|15:30-18:00 | |||
|Individual DatLab analysis + summary | |||
|ORO-MitoFit Lab | |||
|- | |||
|} | |||
::::{| class='wikitable' | |||
|- | |||
|style="width: 10em"|'''Friday 3rd''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Room''' | |||
|- | |||
|09:00 | |||
|SUIT protocols | |||
|MiP''Art'' | |||
|- | |||
|10:30 | |||
|''Coffee'' | |||
| | |||
|- | |||
|11:00 | |||
|Q&A session on HRR and OXPHOS analysis: Design of experimental protocols | |||
|MiP''Art'' | |||
|- | |||
|12:00 | |||
|Lunch break | |||
| | |||
|- | |||
|13:00 | |||
|O2k-Core and O2k-Multisensor | |||
|MiP''Art'' | |||
|- | |||
|14:00 | |||
|DatLab analysis | |||
|ORO-MitoFit Lab | |||
|- | |||
|15:00 | |||
|''Coffee'' | |||
| | |||
|- | |||
|15:30 | |||
|DatLab analysis | |||
|ORO-MitoFit Lab | |||
|- | |||
|16:15 | |||
|The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions | |||
|MiP''Art'' | |||
|- | |||
|17:30 | |||
|Farewell activity | |||
|MiP''Art'' | |||
|- | |||
|} | |||
== General information == | == General information == | ||
::::The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. | ::::The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. | ||
[[File:TRACT logo.png|right|120px|TRACT]] | |||
::::Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR). | |||
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''. | ::::» Information on travel and venue: '''[[IOC Innsbruck]]'''. | ||
== | == Registration == | ||
:::: | ::::Fully booked. | ||
::::''' | ::::'''Maximum number of 6 participants.''' | ||
== Tutors == | |||
<br/> | |||
<gallery mode=default perrow=6 widths="140px" heights="150px"> | |||
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Gnaiger Erich]]''' | |||
File:SumbalovaZ.JPG|'''[[Sumbalova Z|Sumbalova Zuzana]]''' | |||
File:NevesP.JPG|'''[[Neves P|Neves Pedro]]''' | |||
File:PassruggerM.JPG|'''[[Passrugger M|Passrugger Manuela]]''' | |||
File:GarciaL.JPG|'''[[Garcia-Souza LF|Garcia e Souza Luiz Felipe]]''' | |||
File:Velika B.jpg|'''[[Cizmarova B|Cizmarova Beata]]''' | |||
</gallery> | |||
== Participants == | == Participants == | ||
{| class="wikitable" | ::::{| class="wikitable" | ||
|- | |- | ||
! | ! | ||
! Participant | ! Participant | ||
! Institution | ! Institution | ||
|- | |||
| | |||
| [[Bufe A| Anja Bufe]] | |||
| '''[[AT Innsbruck Oroboros ]]:''' Oroboros Instruments, Innsbruck (AT) | |||
|- | |- | ||
| | | | ||
| [[Carvalho E| Eugenia Carvalho]] | | [[Carvalho E| Eugenia Carvalho]] | ||
| '''[[US AR Little Rock | | '''[[US AR Little Rock Borsheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) | ||
|- | |- | ||
| | | | ||
| [[Cotter M| Matthew Cotter]] | | [[Cotter M| Matthew Cotter]] | ||
| '''[[US AR Little Rock | | '''[[US AR Little Rock Borsheim E]]:''' Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) | ||
|- | |- | ||
| | | | ||
| [[Leong I| Ivone Leong]] | | [[Leong I| Ivone Leong]] | ||
| '''SE Stockholm Wikstroem J:''' Karolinska Institute, Stockholm (SE) | | '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE) | ||
|- | |- | ||
| | | | ||
| [[Guan YH| Yue Hugh Guan]] | | [[Guan YH| Yue Hugh Guan]] | ||
| | | Shanghai (CN) | ||
|- | |- | ||
| | | | ||
Line 50: | Line 211: | ||
|- | |- | ||
| | | | ||
| Anna-Maria Wintler | | [[Wintler AM|Anna-Maria Wintler]] | ||
| '''SE Stockholm Wikstroem J:''' Karolinska Institute, Stockholm (SE) | | '''[[SE Stockholm Wikstroem J]]:''' Karolinska Institute, Stockholm (SE) | ||
|- | |- | ||
| | | | ||
Line 64: | Line 225: | ||
:::: | :::: Oroboros Instruments | ||
:::: high-resolution respirometry | :::: high-resolution respirometry | ||
:::: Schöpfstrasse 18 | :::: Schöpfstrasse 18 | ||
Line 75: | Line 236: | ||
== Project support== | == Project support== | ||
:::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol. | :::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol. | ||
:::: [[File:Sponsors.png| 750 px]] | :::: [[File:Sponsors.png| 750 px]] [[File:TRACT logo.png|120px|TRACT]] | ||
== IOC recommended reading == | == IOC recommended reading == | ||
::::» '''[[IOC recommended reading]]''' | ::::» '''[[IOC recommended reading]]''' | ||
::::» '''[[MitoPedia: Respiratory states]]''' | ::::» '''[[MitoPedia: Respiratory states]]''' | ||
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link= | :::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | ||
Line 88: | Line 249: | ||
{{Labeling | {{Labeling | ||
|additional=ORO, IOC, | |instruments=Oxygraph-2k | ||
|additional=ORO, IOC, MitoFit, TRACT, 2017 | |||
}} | }} | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] |
Latest revision as of 10:56, 31 March 2021
MiPNet22.03 IOC119 Innsbruck AT
Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119. |
Oroboros (2017-02-27) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
Programme
Tuesday 28th Topic Room 17:00 - 18:00 Welcome - common aims MiPArt
Wednesday 1st Topic Room 09:00 Mitochondrial and cell research MiPArt 10:00 OroboPOS service and O2k instrumental setup - overview with videoclips MiPArt 10:30 Hands-on: OroboPOS service and O2k instrumental setup MiPArt 12:00 Lunch break 13:00 Instrumental quality control 1: oxygen calibration MiPArt 14:00 Instrumental quality control 2: Instrumental O2 background MiPArt 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 2nd Topic Room 09:00 Introduction to SUIT protocols RP1&RP2 MiPArt 10:00 Coffee 10:30 O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. MiPArt 12:00 Lunch break 13:00 Introduction to DatLab analysis MiPArt 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 3rd Topic Room 09:00 SUIT protocols MiPArt 10:30 Coffee 11:00 Q&A session on HRR and OXPHOS analysis: Design of experimental protocols MiPArt 12:00 Lunch break 13:00 O2k-Core and O2k-Multisensor MiPArt 14:00 DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30 DatLab analysis ORO-MitoFit Lab 16:15 The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions MiPArt 17:30 Farewell activity MiPArt
General information
- The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
- Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR).
- » Information on travel and venue: IOC Innsbruck.
Registration
- Fully booked.
- Maximum number of 6 participants.
Tutors
Participants
Participant Institution Anja Bufe AT Innsbruck Oroboros : Oroboros Instruments, Innsbruck (AT) Eugenia Carvalho US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) Matthew Cotter US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) Ivone Leong SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE) Yue Hugh Guan Shanghai (CN) Vasco Sequeira Oliveira NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL) Anna-Maria Wintler SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE) Evelyn Zacharewicz NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL)
Contact
- Oroboros Instruments
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, MitoFit, TRACT, 2017