OROBOROS INSTRUMENTS

OROBOROS INSTRUMENTS Corp

high-resolution respirometry

Schöpfstr. 18

A-6020 Innsbruck, Austria

Email: mailto:instruments@oroboros.at

» www.oroboros.at

» OROBOROS, AT Innsbruck OROBOROS

A new Quality in Science - we develop, apply and distribute the Oxygraph-2k

unique for high-resolution respirometry combined with O2k-Fluorometry

.. kindle the Light in the Powerhouse of the Cells.

MiPArt by Odra Noel

Get connected

Our service

Are you connected? - This is our network:

• Laboratories in the field of biomedicine and cell biology: O2k-WorldWide MiPNet.
• Scientists and students with an interest in mitochondrial physiology: Applications.
• Our experienced partners in development and production: OROBOROS Partners.
• Scientific expeditions: Science and adventure.

What can we provide to you?

• O2k: the instrument for measurement of cell respiration and key mitochondrial functions: the Oxygraph-2k.
• A concept complete with elaborated protocols, to get started and gain competence in the expanding field of bioenergetics: O2k-Protocols.
• Introductory courses and advanced training: OROBOROS O2k-Workshops.
• High-resolution terminology - matching measurements at high-resolution: MitoPedia Glossaries.

Our instruments

From the Oxygraph-2k to the Power-O2k

O2k: The Oxygraph-2k measures oxygen (dioxygen, O₂) as a gas dissolved in aqueous solutions. With the Oxygraph-2k, a graph is displayed not only of oxygen concentration over time, but of respiration (oxygen consumption, oxygen flux) of the biological sample. The Oxygraph-2k is not used for measurement of oxygen concentration in a tissue, but for high-resolution analysis of respiratory activity of biological samples transferred into the experimental chamber.

Specific substrate-uncoupler-inhibitor titration (SUIT) protocols are applied in studies of cell respiration or mitochondrial activity. In such experiments, quantitative information is obtained on selected biochemical pathways, particularly of the process of oxidative phosphorylation (OXPHOS) in mitochondria and bacteria. Comprehensive OXPHOS analysis leads us beyond the highly informative process of oxygen consumption, when high-resolution respirometry is combined with O2k-MultiSensor Modules to measure simultaneously the production of reactive oxygen species or mitochondrial membrane potential, nitric oxide production, acid-base balance or calcium with O2k-Fluorometry or ion selective electrodes. In the O2k, these bioenergetic parameters are always evaluated in combination with the measurement of oxygen concentration and respiration.

• O2k: The Oxygraph-2k is the 2^nd generation of our high-resolution respirometers. The first model was available since 1992. Development of the new O2k started in 2k (2000) and the first O2k was delivered in 2002.

• O2k: The Oxygraph-2k has two experimental chambers (k: Kammern, German for chambers). Why 2 ml?

• Power-O2k: Several O2k units are operated simultaneously (P1, P2, ..) to combine high-resolution respirometry with high-ouput.

Our profile

Know more about OROBOROS?

• A scientific company located in Austria and distributing the OROBOROS Oxygraph-2k WorldWide: The science company.
• We are specialists in instrumental development and applications in mitochondrial physiology: Top 10 reasons.

• Way beyond an apparatus, we develop a concept connecting scientists: Bioblast MitoPedia.
• OROBOROS: The Logo.

300px

The OROBOROS-Team with visions ..

• We share information from our Office and Mitochondrial Research Laboratory: the OROBOROS-Team.
• As a company of scientists we are supported by artists: the Mitochondrial Physiology Art Gallery - MiPArt.

• Open innovation - building greater ideas: the MitoGlobal Network.

 Gentle Science shapes the world

Stay connected

In an Open-Access approach, OROBOROS provides detailed information on the O2k and modular components, the O2k-Manual, O2k-Protocols and O2k-Publications, the O2k-WorldWide MiPNet, and OROBOROS O2k-Workshops.

» O2k-Info in OroboPedia

» O2k-connected