Difference between revisions of "Octanoylcarnitine"
From Bioblast
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::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O or DMSO, can differ from batch to batch - check Certificate of Analysis): | |||
::::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H<sub>2</sub>O or DMSO. | |||
::::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H<sub>2</sub>O. | |||
::::# Divide into 0.2 mL portions. | ::::# Divide into 0.2 mL portions. | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. |
Revision as of 15:00, 21 November 2019
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
MitoPedia topics:
Substrate and metabolite
Application in HRR
- Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C15H30NO4Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.
- Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 °C.
- O2k manual titrations into 2 mL O2k-chamber