Difference between revisions of "Run DL-Protocol/Set O2 limit"
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{{MitoPedia | {{MitoPedia | ||
|abbr=n.a. | |abbr=n.a. | ||
|description='''Protocols''' can be selected in DatLab (DL7.1) | |description='''DL-Protocols''' can be selected in DatLab (DL7.1) in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of [[Events - DatLab |events]] and [[Marks - DatLab |Marks]]. Instrumental DL-Protocols are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium. DL-Protocols for [[Substrate-uncoupler-inhibitor titration]]s (see [[MitoPedia: SUIT]]) proceed stepwise to activate a sequence of [[coupling control state]]s and [[pathway control state]]s. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump [[TIP2k]] can be programmed to control automatically TIP2k-protocols. Standard DL-Protocols are provided. These can be edited and saved under 'Lab protocols' or under the [[User code - DatLab |User]] logged into DatLab. | ||
}} | }} | ||
{{MitoPedia O2k and high-resolution respirometry | {{MitoPedia O2k and high-resolution respirometry | ||
|mitopedia O2k and high-resolution respirometry=DatLab | |mitopedia O2k and high-resolution respirometry=DatLab | ||
}} | }} | ||
== DL-Protocol == | == DL-Protocol == | ||
After selecting a DL-Protocol, the software indicates for the selected O2k-chamber, which [[Events - DatLab |events]] and [[Marks - DatLab |Marks]] have been set (highlighted in green or turquoise for multiple titration event) and which event is to be set next (highlighted in yellow), with the corresponding mark. | After selecting a DL-Protocol, the software indicates for the selected O2k-chamber, which [[Events - DatLab |events]] and [[Marks - DatLab |Marks]] have been set (highlighted in green or turquoise for multiple titration event) and which event is to be set next (highlighted in yellow), with the corresponding mark. | ||
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== Titrations in SUIT protocols == | == Titrations in SUIT protocols == | ||
::::* Stock concentration of substance B in the titration syringe, ''c''<sub>B,in</sub> [mM] | ::::* Stock concentration of substance B in the titration syringe, ''c''<sub>B,in</sub> [mM] or [µM] as specified. | ||
::::* Titration volume, ''v'' [µl] | ::::* Titration volume, ''v'' [µl] | ||
::::* Chamber volume, ''V'' [µl] | ::::* Chamber volume, ''V'' [µl] | ||
::::* Final concentration, ''c''<sub>B</sub> [mM] | ::::* Final concentration, ''c''<sub>B</sub> [mM] or [µM] as specified. | ||
:::::: ''c''<sub>B</sub> [mM] = ''c''<sub>B,in</sub> [mM] x ''v'' [µl] / ''V'' [µl] | :::::: ''c''<sub>B</sub> [mM] = ''c''<sub>B,in</sub> [mM] x ''v'' [µl] / ''V'' [µl] | ||
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::::» [[MiPNet09.12 O2k-Titrations]] | ::::» [[MiPNet09.12 O2k-Titrations]] | ||
:::: Example: Malate (M), ''c''<sub>M,in</sub> = 800 mM stock; ''v'' = 5 µl titration volume; ''V'' = 2,000 µl chamber volume | :::: Example: Malate (M), ''c''<sub>M,in</sub> = 800 mM stock; ''v'' = 5 µl titration volume; ''V'' = 2,000 µl chamber volume. | ||
:::::: ''c''<sub>M</sub> [mM] = ''c''<sub>M,in</sub> [mM] x ''v'' [µl] / ''V'' [µl] = 800 mM x 5 µl / 2,000 µl = 2 mM | :::::: ''c''<sub>M</sub> [mM] = ''c''<sub>M,in</sub> [mM] x ''v'' [µl] / ''V'' [µl] = 800 mM x 5 µl / 2,000 µl = 2 mM | ||
Revision as of 08:58, 30 June 2017
- high-resolution terminology - matching measurements at high-resolution
Run DL-Protocol/Set O2 limit
Description
DL-Protocols can be selected in DatLab (DL7.1) in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of events and Marks. Instrumental DL-Protocols are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium. DL-Protocols for Substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump TIP2k can be programmed to control automatically TIP2k-protocols. Standard DL-Protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab.
Abbreviation: n.a.
MitoPedia O2k and high-resolution respirometry:
DatLab
DL-Protocol
After selecting a DL-Protocol, the software indicates for the selected O2k-chamber, which events and Marks have been set (highlighted in green or turquoise for multiple titration event) and which event is to be set next (highlighted in yellow), with the corresponding mark.
DL-Protocol will inform you if a titration event is missed or a repetition of a non multiple titration ocurred (highlighted in red).
Titrations in SUIT protocols
- Stock concentration of substance B in the titration syringe, cB,in [mM] or [µM] as specified.
- Titration volume, v [µl]
- Chamber volume, V [µl]
- Final concentration, cB [mM] or [µM] as specified.
- cB [mM] = cB,in [mM] x v [µl] / V [µl]
- Example: Malate (M), cM,in = 800 mM stock; v = 5 µl titration volume; V = 2,000 µl chamber volume.
- cM [mM] = cM,in [mM] x v [µl] / V [µl] = 800 mM x 5 µl / 2,000 µl = 2 mM
