Run DL-Protocol/Set O2 limit

high-resolution terminology - matching measurements at high-resolution

Run DL-Protocol/Set O2 limit

Description

DL-Protocols can be selected in DatLab (DL7.1) in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of Events and Marks. Instrumental DL-Protocols are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium. DL-Protocols for Substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump TIP2k can be programmed to control automatically TIP2k-protocols. A collection of evaluated and tested standard DL-Protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab. Moreover, an automatic warning at low oxygen levels can be set to remind the user to re-oxygenate the chamber in time.

Abbreviation: n.a.

MitoPedia O2k and high-resolution respirometry: DatLab 

Protocol menu

File:Protocol-menu.jpg

Protocol menu

Show DL-Protocol can be ckeck-marked to hide or show the DL-Protocol window. Alternatively, the button (upper left) in the DL-Procotol window can be used to hide or show the window.

Synchronous DL-Protocol events

Set DL-Protocol events

A: Export DL-Protocol

B: Export DL-Protocol applies for chamber B as described above in 'A: Export DL-Protocol'.

Running a DL-Protocol

After selecting a DL-Protocol, the software indicates for the selected O2k-chamber, which events and Marks have been set (highlighted in green or turquoise for multiple titration event) and which event is to be set next (highlighted in yellow), with the corresponding mark.

DL-Protocol will inform you if a titration event is missed or a repetition of a non multiple titration ocurred (highlighted in red).

Titrations in SUIT protocols

• Stock concentration of substance B in the titration syringe, c_B,in [mM] or [µM] as specified.
• Titration volume, v [µl]
• Chamber volume, V [µl]
• Final concentration, c_B [mM] or [µM] as specified.

c_B [mM] = c_B,in [mM] x v [µl] / V [µl]

» MiPNet09.12 O2k-Titrations

Example: Malate (M), c_M,in = 800 mM stock; v = 5 µl titration volume; V = 2,000 µl chamber volume.

c_M [mM] = c_M,in [mM] x v [µl] / V [µl] = 800 mM x 5 µl / 2,000 µl = 2 mM