Difference between revisions of "Run DL-Protocol/Set O2 limit"
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|description='''Protocols''' can be selected in DatLab (DL7.1) which define the sequence of [[Events in DatLab |events] and [[Marks in DatLab |marks]]. Instrumental protocols are used for calibrations and instrumental quality control, typically without experimental smaple in the incubation medium. [[Substrate-uncoupler-inhibitor titration]] protocols (see [[MitoPedia: SUIT]]) proceed stepwise to activate a sequence of [[coupling control state]]s and [[pathway control state]]s. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump [[TIP2k]] can be programmed to control automatically TIP2k-protocols. Standard protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab. | |description='''Protocols''' can be selected in DatLab (DL7.1) which define the sequence of [[Events in DatLab |events] and [[Marks in DatLab |marks]]. Instrumental protocols are used for calibrations and instrumental quality control, typically without experimental smaple in the incubation medium. [[Substrate-uncoupler-inhibitor titration]] protocols (see [[MitoPedia: SUIT]]) proceed stepwise to activate a sequence of [[coupling control state]]s and [[pathway control state]]s. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump [[TIP2k]] can be programmed to control automatically TIP2k-protocols. Standard protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab. | ||
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{{MitoPedia O2k and high-resolution respirometry | {{MitoPedia O2k and high-resolution respirometry | ||
|mitopedia O2k and high-resolution respirometry=DatLab | |mitopedia O2k and high-resolution respirometry=DatLab | ||
}} | }} | ||
== Protocol editor == | == Protocol editor == | ||
:::: The sequence of Events and Marks can be edited with names, definitions, concentrations and titration volumes. After a protocol is edited and saved under a defined name, the protocol has to be selected and assigned to one or both O2k-chambers. | :::: The sequence of Events and Marks can be edited with names, definitions, concentrations and titration volumes. After a protocol is edited and saved under a defined name, the protocol has to be selected and assigned to one or both O2k-chambers. | ||
Revision as of 16:16, 13 November 2016
{{MitoPedia |abbr=n.a. |description=Protocols can be selected in DatLab (DL7.1) which define the sequence of [[Events in DatLab |events] and marks. Instrumental protocols are used for calibrations and instrumental quality control, typically without experimental smaple in the incubation medium. Substrate-uncoupler-inhibitor titration protocols (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump TIP2k can be programmed to control automatically TIP2k-protocols. Standard protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab. }}
MitoPedia O2k and high-resolution respirometry: DatLab
Protocol editor
- The sequence of Events and Marks can be edited with names, definitions, concentrations and titration volumes. After a protocol is edited and saved under a defined name, the protocol has to be selected and assigned to one or both O2k-chambers.
- A. Instrumental protocols: calibration and instrumental O2 background test
- B. SUIT protocols: linked to titration solutions
- C. TIP2k-protocols.
