Difference between revisions of "Run DL-Protocol/Set O2 limit"
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== DL-Protocol == | == DL-Protocol == | ||
After selecting a DL-Protocol, the software indicates for the selected O2k-chamber, which [[Events - DatLab |events]] and [[Marks - DatLab |Marks]] have been set (highlighted in green or turquoise for multiple titration event) and which event is to be set next (highlighted in yellow), with the corresponding mark. | :::: After selecting a DL-Protocol, the software indicates for the selected O2k-chamber, which [[Events - DatLab |events]] and [[Marks - DatLab |Marks]] have been set (highlighted in green or turquoise for multiple titration event) and which event is to be set next (highlighted in yellow), with the corresponding mark. | ||
DL-Protocol will inform you if a titration event is missed or a repetition of a non multiple titration ocurred (highlighted in red). | :::: DL-Protocol will inform you if a titration event is missed or a repetition of a non multiple titration ocurred (highlighted in red). | ||
== Titrations in SUIT protocols == | == Titrations in SUIT protocols == |
Revision as of 11:00, 11 July 2017
Description
DL-Protocols can be selected in DatLab (DL7.1) in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of Events and Marks. Instrumental DL-Protocols are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium. DL-Protocols for Substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump TIP2k can be programmed to control automatically TIP2k-protocols. Standard DL-Protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab.
Abbreviation: n.a.
MitoPedia O2k and high-resolution respirometry:
DatLab
DL-Protocol
- DL-Protocol will inform you if a titration event is missed or a repetition of a non multiple titration ocurred (highlighted in red).
Titrations in SUIT protocols
- Stock concentration of substance B in the titration syringe, cB,in [mM] or [µM] as specified.
- Titration volume, v [µl]
- Chamber volume, V [µl]
- Final concentration, cB [mM] or [µM] as specified.
- cB [mM] = cB,in [mM] x v [µl] / V [µl]
- Example: Malate (M), cM,in = 800 mM stock; v = 5 µl titration volume; V = 2,000 µl chamber volume.
- cM [mM] = cM,in [mM] x v [µl] / V [µl] = 800 mM x 5 µl / 2,000 µl = 2 mM