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=== Part 1.1: OroboPOS service and O2k instrumental set up === ...s://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS]

...rer-Bar\white PVDF\15x6 mm|PVDF Stirrer-Bars]], 2 [[PEEK]] O2k-Stoppers, [[OroboPOS-Connector]]s for O2k-series A-I and NextGen-O2k series XA (attached to the

...ight|180px]]'''OroboPOS-Connector''' (blue POM), with male connection to [[OroboPOS]] head (POS) and with cable and male plug fitting into [[O2k-Main Unit]]. |info=[https://www.oroboros.at/index.php/product/orobopos-connector/ '''Product details and purchase information''']

...on]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]] ::::# Unplug the [[POS-Connector]] from the O2k-main unit and unscrew the [[OroboPOS]] from the [[POS-Connector]]. Screw the POS to the blue part of the perspex

...####''' '''[[Oroboros O2k]]''' / '''[[O2k-Catalogue: OroboPOS components|OroboPOS]]'''

...b>2</sub>S₂O₄), for zero-oxygen calibration of the [[OroboPOS]].

*Copper-Block with windows to 2 O2k-Chambers *2 Amperometric OroboPOS Plugs

...the [[OroboPOS|POS]] to the 2-mL [[O2k-chamber]], and keeping the SmartPOS/OroboPOS-Connector in a fixed position for sealing the O2k-chamber with the [[POS-Se |info=[https://www.oroboros.at/index.php/product/orobopos-holder/ '''Product details and purchase information''']

|abbr=H₂O ...iH₂O) with various combination purification methods. When H₂O is mentioned without further specification in published protocols, i

|description=The 2-chamber design of the O2k helps to '''search for defective O2k components'' ::#[https://www.youtube.com/watch?v=ju-qZ2sz5ro how to disassemble the OroboPos ]

...omponent of [[MitoPedia: Oroboros QM |Oroboros Quality Management]]. The [[OroboPOS]] test is described in detail in [[MiPNet06.03 POS-calibration-SOP]], is pe ::::The O2 sensor test does not only serve to evaluate the function of the OroboPOS, but many other components of the O2k have to function according to specifi

...tube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use] ...Gzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]

=== Part 1.1: OroboPOS service and O2k instrumental === ...s://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 O2k-Videosupport: OroboPOS from Perspex housing]

...ressure and electric current [Amp] (amperometric mode of operation). The [[OroboPOS]] is the POS applied in the [[Oroboros O2k]]. |info=[[OroboPOS]]

...brane application. Since the O2k-Series G-0075 membrane ring holder have a 2 mm extended ridge for a better grip. |info=[https://www.oroboros.at/index.php/product/orobopos-mounting-tool/ '''Product details and purchase information''']

:::: The '''O₂ sensor and O2k chamber tests''' are important to eliminate instrument === 1. O₂ sensor test ===

...k]] is transmitted from the electrochemical polarographic oxygen sensor ([[OroboPOS]]) for each of the two O2k-chambers to [[DatLab]]. The primary signal is a ...[µM], but the numbers shown as a default are different from the real O₂ concentration. The non-calibrated default values are calculated on th

::::* When a new [[OroboPOS-Connector]] is used. ...as % of the raw signal at air saturation. New POS have a zero signal below 2 % of the signal at air saturation. For accurate calibration, the zero signa

...roboPOS]]. Two [[O2k-Chamber Holder]]s, two [[OroboPOS-Holder]]s and two [[OroboPOS-Connector]]s are attached to the O2k-Main Unit for transport. ...PNet22.11_O2k-FluoRespirometer_manual]]) and store the O2k-Chambers in the OroboPOS-Service Kit box.

...us medium and ensure a stable signal of the polarographic oxygen sensor ([[OroboPOS]]) placed in a position of maximum current of the medium.

...right|180px]]'''Volume-Calibration Ring''' (A or B) PVDF for O2k-Stoppers (2-mL [[O2k-chamber]]), white; attached to the [[Stopper\black PEEK\conical Sh ::::* Loosen the stopper fixation rings (A) and (B) with the Allen key (OroboPOS-Service Kit).

...(O2k Quality Control)]] for the [[O2k-Open_Support#1._O2_sensor_test|O₂ sensor test]], the stirrer test is performed in the 'open' chamber in

|description=What are potential causes for '''different O₂ fluxes in the left and right chamber'''? ...ocedure described in the section '''Calibration and quality control of the OroboPOS (O2k-SOP)''' in [[O2_calibration_-_DatLab#Calibration_and_quality_control|

...mber''': Duran® glass polished, with standard operation volumes (''V'') of 2.0 mL or 0.5 mL (small chamber volume in the [[O2k-sV-Module]], 12 mm inner ...''' - [https://www.oroboros.at/index.php/product/o2k-chamber/ O2k-Chamber (2.0 mL)], [https://www.oroboros.at/index.php/product/o2k-chamber-sv/ O2k-Cham

...esolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhi |OroboPOS service and O2k instrumental setup - overview with videoclips

...tatus line|status line]], besides the DatLab version number (bottom), and (2) in windows [[O2k control]] [F7] and [[O2k configuration]]. ::::* Type of [[OroboPOS-Connector]]

::::::::''16:30 - 17:15'' O2k setup (team 1 and 2); OroboPOS service (team 3 and 4) ...17:15 - 18:00'' O2k setup (team 3 and 4); OroboPOS service (team 1 and 2)

== Electrochemical NO and H₂O₂ sensors == ...sensors. All amperometric sensors (NO, H₂S, H₂O₂) that have the same connection can be used with the O2k.

...k-Fluorometry for measurement of hydrogen peroxide, ATP production, Ca²⁺ uptake capacity, mitochondrial membrane potential with fluorometric ...ality controls 1 ([[MiPNet06.03 POS-calibration-SOP |O2 sensor test]]) and 2 ([[MiPNet14.06 Instrumental O2 background |O2k-chamber test]]), which are e

...you know that you can set a warning alarm for the lower limit of the O₂ concentration in DatLab 7? '''<br> - »[[Run_DL-Protocol/Set_O2_limit#Protocol_menu | '''Set O₂ limit in DatLab''']]« <br>

|Introduction - OroboPOS service and O2k instrumental setup - overview with videoclips |Hands-on: OroboPOS service and O2k instrumental setup

|OroboPOS service and O2k instrumental setup - overview with videoclips |'''Hands-on''': OroboPOS service and O2k instrumental setup

...esolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhi |2.77

[[File:Distributor training 2.jpeg|500px|right|Distributor training]] :::: 16:00 - 17:00 General questions (OroboPOS-Connector, quality control…)

...-wide distibution of the O2k. With completion of the development of DatLab 2 in cooperation with Dr. Michael Reck, the term "high-resolution respirometr ::::In '''[[Oroboros 1997| 1997]]''' Oroboros released DatLab 2, setting a new standard in software-supported high-resolution respirometry.

...tration]]s in [[MiPNet14.06 Instrumental O2 background |instrumental O₂ background tests]]. It is not recommended to use dithionite in experi |chem formula=Na₂S₂O₄

:::: Minimum duration: 2 days ...from instrumental setup and service of the polarographic oxygen sensor ([[OroboPOS]]) for instrumental quality control system, which is an essential component

|abbr=''J''°_O₂ ...e range of experimental oxygen levels by a standardized instrumental O₂ background test. The oxygen regime from air saturation towards zero o

...oxygen partial pressure at the temperature defined by the experimenter; (''2'') zero oxygen calibration; (''3'') high stability of the POS signal tested ...ring an instrumental calibration run. '''Step <span style="background:red">2</span>''': Click on <span style="background:lightgrey">Calibrate and copy t

''' [[OroboPOS| Polarographic oxygen sensor (POS)]] and O2k service''' ...potential ([[Safranin]]), ATP production ([[Magnesium green]]), and Ca²⁺ ([[Calcium green]]). Further O2k-MultiSensor applications are covere

...s of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essentia ...ys prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.

::::* Instrumental setup, chamber assembly, OroboPOS – sensor service, quality control (group Carmen Sandi). :::: Design of experimental protocol - day 2.

...oduction of the Oroboros O2k was preceeded by instrumental developments [1,2] including the Twin-Flow Microrespirometer [3] for simultaneous application ...solution respirometry [9-12]. With completion of the development of DatLab 2 Dr. Michael Reck), the term "high-resolution respirometry" [HRR] was coined

...n of respiratory rate by cell count yields oxygen [[flow]] ''I''_O₂ expressed in units [amol·s^-1·x^-1] (=10< ...ibrated experimental volume of ''V''=0.5 mL or 2.0 mL using the 0.5-mL and 2.0-mL chamber, respectively. The corresponding corrections, however, depend

|''' Why is it important to check the H₂O₂ sensitivity when conducting an Amplex UltraRed (AmR) assay? ...osition, added chemicals and sample, requiring multiple H₂O₂ calibrations throughout the measurement. After the initial AmR calibr

Day 2: SUIT protocol for comparative mitochondrial physiology - a pilot experimen ...ive to the Amazon, and is among the largest freshwater fish in the world (>2.5 m). As an adaptation to the seasonal hypoxic conditions, Arapaima has dev

File:IOC115 (2).jpg|Full in action: Participants practising O2k applications File:IOC115 (4).jpg|Participants practising the OroboPOS sensor service

...nditioning solution 1''' (dissolved in 100 mM KCl solution) for use in '''2-mL O2k-chamber''': ...nditioning solution 2''' (dissolved in 100 mM KCl solution) for use in '''2-mL O2k-chamber''':

...cle/view/gnaiger_2020_mitopathways Gnaiger 2020 BEC MitoPathways - Chapter 2.4]« <br> ...ndaries. Therefore, a short-chain, membrane-permeable CoQ mimetic, CoQ₂ is used as a probe which reacts with the mitochondrial respiratory Co

...you know that you can set a warning alarm for the lower limit of the O₂ concentration in DatLab 7? '''<br> - » [[Run_DL-Protocol/Set_O2_limit#Protocol_menu | Set O₂ limit]] in DatLab « <br>

|'''Mountain slopes can be difficult, but O₂ slopes can be smooth with DatLab'''<br> ...ints, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.

Prepare your TIP2k for Instrumental O₂ background: <br> ...v=VV9pRcK076k O2k-Videosupport: Working with the TIP2k (instrumental O₂ background)]'''«<br>