Difference between revisions of "TPP+ inhibitory effect"
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{{ | {{MitoPedia | ||
|description=A major task in establishing a procedure for measurement of [[mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The '''TPP<sup>+</sup> inhibitory effect''' (this also applies to TPMP<sup>+</sup> and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP<sup>+</sup>, and for restriction of experimental TPP<sup>+</sup> concentrations below the inhibitory range. | |||
|info=[[MiPNet14.05]] | |||
}} | |||
{{Keywords: Force and membrane potential}} | |||
{{MitoPedia methods | |||
{{MitoPedia | |mitopedia method=Respirometry | ||
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}} | }} | ||
{{ | {{MitoPedia topics | ||
| | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Latest revision as of 00:03, 18 February 2020
Description
A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP+ inhibitory effect (this also applies to TPMP+ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range.
Reference: MiPNet14.05
- Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Fundamental relationships
- mt-Membrane potential and protonmotive force
- O2k-Potentiometry
- » O2k-Catalogue: O2k-TPP+ ISE-Module
- » O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
- » TPP - O2k-Procedures: Tetraphenylphosphonium
- » Specifications: MiPNet15.08 TPP electrode
- » Poster
- » Unspecific binding of TPP+
- » TPP+ inhibitory effect
- O2k-Potentiometry
- O2k-Fluorometry
- » O2k-Catalogue: O2k-FluoRespirometer
- » O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- » Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
- » TMRM - O2k-Procedures: TMRM
- O2k-Fluorometry
- O2k-Publications
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor