Appaix 2000 Biochim Biophys Acta
|Appaix F, Minatchy M-N, Riva-Lavieille C, Olivares J, Antonsson B, Saks VA (2000) Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited. Biochim Biophys Acta 1457:175-81.|
Abstract: This paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (γ) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes.
• O2k-Network Lab: FR Grenoble Saks VA
Tissue;cell: Heart Preparation: Permeabilized tissue, Isolated mitochondria Enzyme: Complex IV;cytochrome c oxidase
Coupling state: OXPHOS, ET