Armstrong 2010 J Comp Physiol B

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Armstrong C, Staples JF (2010) The role of succinate dehydrogenase and oxaloacetate in mitochondrial metabolism during mammalian hibernation and arousal. J Comp Physiol B 180:775-83.

» PMID: 20112024

Armstrong C, Staples JF (2010) J Comp Physiol B

Abstract: Hibernation elicits a major reduction in whole-animal O2 consumption that corresponds with active suppression of liver mitochondrial electron transport capacity at, or downstream of, succinate dehydrogenase (SDH). During arousal from the torpor phase of hibernation this suppression is reversed and metabolic rates rise dramatically. In this study, we used the 13-lined ground squirrel (Ictidomys tridecemlineatus) to assess isolated liver mitochondrial respiration during the torpor phase of hibernation and various stages of arousal to elucidate a potential role of SDH in metabolic suppression. State 3 and state 4 respiration rates were seven- and threefold lower in torpor compared with the summer-active and interbout euthermic states. Respiration rates increased during arousal so that when body temperature reached 30 degrees C in late arousal, state 3 and state 4 respiration were 3.3- and 1.8-fold greater than during torpor, respectively. SDH activity was 72% higher in interbout euthermia than in torpor. Pre-incubating with isocitrate [to alleviate oxaloacetate (OAA) inhibition] increased state 3 respiration rate during torpor by 91%, but this rate was still fourfold lower than that measured in interbout euthermia. Isocitrate pre-incubation also eliminated differences in SDH activity among hibernation bout stages. OAA concentration correlated negatively with both respiration rates and SDH activity. These data suggest that OAA reversibly inhibits SDH in torpor, but cannot fully account for the drastic metabolic suppression observed during this hibernation phase.

Keywords: Hibernation, Ground squirrel (Ictidomys tridecemlineatus), Arousal, Mitochondria, Succinate dehydrogenase, Oxaloacetate

O2k-Network Lab: CA London Staples JF


Labels:


Organism: Other mammals  Tissue;cell: Liver  Preparation: Isolated mitochondria  Enzyme: Complex II;succinate dehydrogenase, TCA cycle and matrix dehydrogenases  Regulation: Substrate  Coupling state: LEAK