Beer-Lambert law

From Bioblast

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Beer-Lambert law


This law states that the transmittance (T) of light though a sample is given by: T = e-Ξ΅bc, where Ξ΅ is the molar extinction coefficient, b is the pathlength of the light through the cuvette (in mm) and c is the concentration of the pigment in the sample (in mM). Transforming this equation, it can be seen that the absorbance of light (A) is simply given by A = Ξ΅bc.

Abbreviation: B-L law

MitoPedia methods: Spectrophotometry 

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