Briones-Herrera 2018 Food Chem Toxicol

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Briones-Herrera A, Avila-Rojas SH, Aparicio-Trejo OE, Cristóbal M, León-Contreras JC, Hernández-Pando R, Pinzón E, Pedraza-Chaverri J, Sánchez-Lozada LG, Tapia E (2018) Sulforaphane prevents maleic acid-induced nephropathy by modulating renal hemodynamics, mitochondrial bioenergetics and oxidative stress. Food Chem Toxicol 115:185-97.

» PMID: 29548851

Briones-Herrera A, Avila-Rojas SH, Aparicio-Trejo OE, Cristobal M, Leon-Contreras JC, Hernandez-Pando R, Pinzon E, Pedraza-Chaverri J, Sanchez-Lozada LG, Tapia E (2018) Food Chem Toxicol

Abstract: Maleic acid (MA)-induced nephropathy that is characterized by proteinuria, glycosuria, phosphaturia and a deficient urinary acidification and concentration. Sulforaphane (SF) is an indirect antioxidant that shows nephroprotective effects. The aim of the present work was to test the pre-treatment with SF against the MA-induced nephropathy.

Wistar rats (230-260 g) were separated in the following groups: control, MA (which received 400 mg/kg of MA), SF + MA (which received MA and 1 mg/kg of SF each day for four days) and SF (which only received SF). MA induced proteinuria, an increase in urinary excretion of N-acetyl-β-d-glucosaminidase, and a decrease in plasma glutathione peroxidase activity, renal blood flow, and oxygenation and perfusion of renal cortex. All these impairments correlated with higher levels of oxidative damage markers and exacerbated superoxide anion production on renal cortex. Moreover, MA impaired mitochondrial bioenergetics associated to complex I, mitochondrial membrane potential and respiratory control index and increased the mitochondrial production of hydrogen peroxide. Further it disrupted mitochondrial morphology. SF prevented all the above-described alterations.

In conclusion, the protective effect of SF against MA-induced nephropathy is associated with preservation of mitochondrial bioenergetics, amelioration of oxidative stress and improvement of renal hemodynamics and renal cortex oxygenation.

Keywords: Acute kidney injury, Kidney, Maleate, Maleic acid, Renal, Sulforaphane Bioblast editor: Plangger M O2k-Network Lab: MX Mexico City Pedraza Chaverri J


Labels: MiParea: Pharmacology;toxicology  Pathology: Other 

Organism: Rat  Tissue;cell: Kidney  Preparation: Isolated mitochondria 


Coupling state: LEAK, OXPHOS  Pathway: N, S, NS  HRR: O2k-Fluorometer 

2018-09, Amplex UltraRed