Chang 2017 FASEB J
|Effects of cycling exercise on mitochondrial function and efficacy of peripheral blood mononuclear cells under hypoxic condition in sedentary men.|
Link: Open Access
Dysfunction of peripheral blood mononuclear cells (PBMCs) has been found in hypoxemic patients suffering from cardiopulmonary disorders or those exposed to hypoxic environments such as high altitudes. The mitochondrial bioenergetics of PBMCs serves a critical role in PBMC-related immune function. The purpose of this study is to elucidate whether cycling exercise affect mitochondrial function and efficacy in PBMCs under hypoxic stress. Twelve sedentary males engaged in moderate exercise (60% VO2max) for 40 min while exposed to 12%O2 (HE) in a normobaric hypoxia chamber. Before and immediately after HE, Substrate-Uncoupler-Inhibitor Titrations (SUIT) protocols and TMRE, a mitochondrial membrane potential fluorescent dye, were applied on PBMCs and analyzed by a high resolution respirometer (Oroboros Oxygraph-2k). This study can be summarized as follows: (1) although no differences in routine or maximal mitochondrial O2 consumption rate, the HE heightened mitochondrial membrane potential in intact PBMCs and (2) the HE depressed pyruvate-/glutamate-dependent complex I (NADH-related) O2 consumption rate in mitochondria of permeabilized PBMCs. Hence, we conclude that the HE is insufficient to influence maximal oxidative phosphorylation and electron transport capacity of mitochondria despite of depressing the NADH-related mitochondrial efficacy in PBMCs.
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Tissue;cell: Blood cells Preparation: Intact cells, Permeabilized cells
Coupling state: ROUTINE Pathway: N HRR: Oxygraph-2k