Chatfield 2019 JACC Basic Transl Sci

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Chatfield KC, Sparagna GC, Chau S, Phillips EK, Ambardekar AV, Aftab M, Mitchell MB, Sucharov CC, Miyamoto SD, Stauffer BL (2019) Elamipretide improves mitochondrial function in the failing human heart. JACC Basic Transl Sci 4:147-57.

» PMID: 31061916 Open Access

Chatfield KC, Sparagna GC, Chau S, Phillips EK, Ambardekar AV, Aftab M, Mitchell MB, Sucharov CC, Miyamoto SD, Stauffer BL (2019) JACC Basic Transl Sci

Abstract: Negative alterations of mitochondria are known to occur in heart failure (HF). This study investigated the novel mitochondrial-targeted therapeutic agent elamipretide on mitochondrial and supercomplex function in failing human hearts ex vivo. Freshly explanted failing and nonfailing ventricular tissue from children and adults was treated with elamipretide. Mitochondrial oxygen flux, complex (C) I and CIV activities, and in-gel activity of supercomplex assembly were measured. Mitochondrial function was impaired in the failing human heart, and mitochondrial oxygen flux, CI and CIV activities, and supercomplex-associated CIV activity significantly improved in response to elamipretide treatment. Elamipretide significantly improved failing human mitochondrial function.

Keywords: ADP, Adenosine diphosphate, BN-PAGE, Blue native polyacrylamide gel electrophoresis, C, Mitochondrial respiratory complex, FCCP, Carbonyl cyanide p-trifluoromethoxyphenylhydrazone, HF, Heart failure, RCR, Respiratory control ratio, SC CCF, Supercomplex coupling control factor, Heart failure, High-resolution respirometry, Mitochondrial-targeted compounds, Supercomplex Bioblast editor: Plangger M O2k-Network Lab: US CO Aurora Sparagna GC


Labels: MiParea: Respiration, Pharmacology;toxicology  Pathology: Cardiovascular 

Organism: Human  Tissue;cell: Heart  Preparation: Permeabilized tissue  Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Supercomplex 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS  HRR: Oxygraph-2k 

Labels, 2019-05