Costa 2013 Cell Death Dis

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Costa AC, Loh SH, Martins LM (2013) Drosophila Trap1 protects against mitochondrial dysfunction in a PINK1/parkin model of Parkinson's disease. Cell Death Dis 4:e467.

» PMID: 23328674 Open Access

Costa AC, Loh SH, Martins LM (2013) Cell Death Dis

Abstract: Mitochondrial dysfunction caused by protein aggregation has been shown to have an important role in neurological diseases, such as Parkinson's disease (PD). Mitochondria have evolved at least two levels of defence mechanisms that ensure their integrity and the viability of their host cell. First, molecular quality control, through the upregulation of mitochondrial chaperones and proteases, guarantees the clearance of damaged proteins. Second, organellar quality control ensures the clearance of defective mitochondria through their selective autophagy. Studies in Drosophila have highlighted mitochondrial dysfunction linked with the loss of the PTEN-induced putative kinase 1 (PINK1) as a mechanism of PD pathogenesis. The mitochondrial chaperone TNF receptor-associated protein 1 (TRAP1) was recently reported to be a cellular substrate for the PINK1 kinase. Here, we characterise Drosophila Trap1 null mutants and describe the genetic analysis of Trap1 function with Pink1 and parkin. We show that loss of Trap1 results in a decrease in mitochondrial function and increased sensitivity to stress, and that its upregulation in neurons of Pink1 mutant rescues mitochondrial impairment. Additionally, the expression of Trap1 was able to partially rescue mitochondrial impairment in parkin mutant flies; and conversely, expression of parkin rescued mitochondrial impairment in Trap1 mutants. We conclude that Trap1 works downstream of Pink1 and in parallel with parkin in Drosophila, and that enhancing its function may ameliorate mitochondrial dysfunction and rescue neurodegeneration in PD.

Keywords: Parkinson’s disease, Drosophila, PTEN-induced putative kinase 1 (PINK1), TNF receptor-associated protein 1 (TRAP1), Stress

O2k-Network Lab: UK Leicester Martins LM

Labels: MiParea: Respiration, Genetic knockout;overexpression, mt-Medicine  Pathology: Parkinson's 

Organism: Drosophila 

Preparation: Permeabilized tissue  Enzyme: Complex I, Complex II;succinate dehydrogenase  Regulation: Inhibitor  Coupling state: OXPHOS, ET  Pathway: N, S  HRR: Oxygraph-2k