Dihydroethidium

From Bioblast


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Dihydroethidium

Description

Dihydroethidium (also called hydroethidine) is a cell permeant fluorescent probe used to analyse superoxide presence. It is a reduced form of ethidium that presents blue fluorescence, and after oxidation by superoxide becomes able to intercalate DNA and emits red fluorescence (excitation wavelength ~518–535 nm, emission ~605–610 nm). It has been used to detect superoxide by HPLC and by fluorescence microscopy.

Abbreviation: DHE

Reference: Laurindo 2008 Methods Enzymol, Kalivendi 2003 Biochem J

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